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1、NEBufferActivityChartforRestrictionEnzymesNewEnglandBiolabsprovidesacolor-coded10XNEBufferwitheveryrestrictionendonucleasetoensure100%activity.Tohelpselectthebestconditionsfordoubledigests,thischartshowstheoptimal(supplied)NEBufferandapproximateactivityinthefourstandardNEBuffersfo
2、reachenzyme.IfBSAissuppliedwithanenzyme,itisincludedinallNEBufferactivityreactions.Inaddition,thetablealsoshowsrecommendedreactiontemperature,heatinactivationtemperatureandrecommendeddiluentbuffer.Pleaseclickhereforadditionalinformationabout:UniqueNEBufferActivity??
3、DoubleDigestio
4、n
5、HeatInactivation
6、Activityat37°C
7、DiluentBuffers
8、Time-SaverEnzymes
9、HighFidelity(HF)RestrictionEnzymesThischarthasbeenupdatedtoreflectthechangeof54restrictionenzymestoNEBuffer4onNov.1,2008.ClickfortheChartLegend,IconDescriptionA
10、B
11、C
12、D
13、E
14、F
15、H
16、I
17、K
18、M
19、N
20、P
21、R
22、S
23、T
24、X
25、Z
26、EnzymeSuppliedNEBuffe
27、r%ActivityinNEBuffersHeatInactiva-tion°CIncuba.Temp.°CDiluent1234AatIINEBuffer4050501006537AAcc65INEBuffer3+BSA1075100256537AAccINEBuffer45050101008037AAciINEBuffer32550100506537AAclINEBuffer4+BSA10100100No37BAcuINEBuffer4+SAM50100501006537BAfeINEBuffer42550251006537AAflIINEBuffer
28、4+BSA50100251006537AAflIIINEBuffer3+BSA2575100508037BAgeINEBuffer11005010756537CAhdINEBuffer4+BSA257501006537AAleINEBuffer41025101006537AAluINEBuffer4100100751006537BAlwINEBuffer450100101006537AAlwNINEBuffer450100501006537AApaINEBuffer4+BSA255001006525AApaLINEBuffer4+BSA1001001010
29、0No37AApeKINEBuffer3257510050No75BApoINEBuffer3+BSA1075100758050AAscINEBuffer4010101006537AAseINEBuffer3NR75100ddNR6537BAsiSINEBuffer4+BSA501001001008037BAvaINEBuffer41075101008037AAvaIINEBuffer45075101006537AAvrIINEBuffer4100100501008037BEnzyme????topSuppliedNEBuffer%inB1%inB2%in
30、B3%inB4HeatInactiva.Incuba.Temp.DiluentBaeGINEBuffer11007510506537ABaeINEBuffer4+BSA+SAM50100501006525ABamHINEBuffer3+BSA75100100dd100No37ABamHI-HF?NEBuffer41005010100No37ABanINEBuffer4+BSA50100501006537ABanIINEBuffer410010501008037ABbsINEBuffer210010025756537BBbvCINEBuffer4501001
31、01008037ABbvINEBuffer210010025756
