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1、VirusResearch,11(1988)141-150141ElsevierVRR00440Aminoacidswithinhypervariableregion1ofaviancoronavirusIBV(Massachusettsserotype)spikeglycoproteinareassociatedwithneutralizationepitopesDavidCavanagh,PhilipJ.DavisandA.P.AdrianMockettAFRCInstituteforAnimalHealt
2、h,HoughtonLaboratory,Houghton,Huntingdon,Cambridgeshire,U.K.(Accepted13May1988)Thespikeglycoprotein(S)geneofIBVcodesforaprecursorproteinwhichiscleavedintotheN-terminalSlandC-terminalS2glycopolypeptides.TheSlglycopolypeptide,whichinducesneutralizingantibody,c
3、omprisesapproximately520aminoacidresidues.WehavedeterminedthenucleotidesequenceofSlofsevenstrainsoftheMassachusetts(Mass)serotypeandthefirst337basesoftwoadditionalMassstrains.DespitethefactthatthestrainshadbeenisolatedoverthreedecadesinEuropeandtheU.S.A.ther
4、ewasonly4%baseand6%aminoacidvariationwithinthegroup.Nearlyonethirdofthe32aminoaciddifferencesinSlwereintwohypervariableregions(HVRs1and2)comprisingresidues38-51and99-115,identifiedbyNiestersetal.(1986),showingthatHVRs1and2areafeatureoftheMassserotype.Aminoac
5、idvariationwithinHVRs1and2was29%and40%respectively.Fivevaccinestrainscouldbedistinguishedfromeachotherbysequencingofthefirst337nucleotides.VariantsofM41whichresistedneutraliza-tionbytwomonoclonalantibodies(Al3andA38)hadthesame,singlebasechangeatposition134,r
6、esultinginsubstitutionofprolineresidue45byhistidine.ThisindicatesthatresidueswithinHVR1areassociatedwithepitopeswhichinduceneutralizingantibody.CoronavirusIBV;Neutralization;Epitope;VariationCorrespondenceto:D.Cavanagh,AFRCInstituteforAnimalHealth,HoughtonLa
7、boratory,Houghton,Huntingdon,Cambridge&irePE17ZDA,U.K.0168-1702/88/$03.5001988ElsevierSciencePublishersB.V.(BiomedicalDivision)142IntroductionExtensiveantigenicvariationisafeatureofIBVandnewserotypes,basedonneutralizationtests,havebeendetectedfrequently(Cook
8、andHuggins,1986).Onlyrecentlyhasthemolecularbasisforthisvariationbeensought.Mostattentionhasbeenfocusedonthespikeglycoprotein(S),themajorinducerofneutralizingantibody(Cavanaghetal.,1984,1986a),w
