資源描述：
《冠狀病毒Coronavirusl論文-1987 Calfhood Coronavirus Enterocolitis_ A Clue to the Etiology of Winter Dysentery.pdf》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、Vet.Pathol.24564-567(1987)BRIEFCOMMUNICATIONSCalfhoodCoronavirusEnterocolitis:ACluetotheEtiologyofWinterDysenteryH.J.VANQUININGEN,L.H.KHAIRALLAH,V.G.SASSEVILLE,M.S.WYAND,ANDJ.E.POSTRecentlywepublishedareviewofwinterdysenteryanddestructionofepithelialcellsproducedtw
2、ocharacteristicele-documentedfindingsinspontaneousandexperimentaldis-ments:amoth-eatenappearanceofthemucosaatlowmag-easeinNewEnglanddairycattle.’OThehistologicfeaturesofnificationandstrikingnuclearpyknosisandcytoplasmichya-winterdysenterywerecharacterizedforthefirs
3、ttime.Ale-linedropletsathighmagnification(Fig.1).Acowthatdiedsionofthecolon,particularlythespiralcolon,wasrecog-hadwidespreadchangesofthistypeaswellasreparativenized.Itconsistedofpyknosis,karyorrhexis,granularde-dilatedcrypts.Thesefeatureswererecognizedasuniqueandg
4、eneration,hydropicdegeneration,andhyalinedropletsuggestiveof“virus-inducedenterocolitis.”degenerationofcryptepithelialcells.ThisscatteredfocalWhileourworkwasinprogress,investigatorsfromJapan9Fig.1.Coloniccryptfromspontaneouscaseofwinterdysentery.Epithelialcellswith
5、variousdegenerativeandnecroticchanges.Withincryptlumen,dyingcellscontainkaryorrhecticnucleiandhyalinedroplets.HE.Fig.2.Histologicsectionofspiralcolonfromcalfwithcoronavirusenterocolitis.Extensivecryptepithelialcellpyknosis,karyorrhexis,lysis,andregenerativehyperpla
6、siaidenticaltothatoccumnginwinterdysentery.Lossofcryptsleukocytosis,tatteredsurfaceepithelium,andgranulocyticexudateatthesurface.HE.564BriefCommunications565Fig.3.Negativelystainedcoronavirusparticlesinintestinalcontentsfromaffectedcalf.Sizefrom95-115nm,130-150nmwi
7、thpeplomeres.Bar=116nm.Fig.4.Numerouscoronavirusparticlesand“virusfactories”withindegenerateepithelialcellfromdamagedcolonicmucosaofcalf.Formalin-fixedtissue,virusparticles60nmindiameter.Bar=200nm.Fig.5.Numerouscoronavirusparticlesand“virusfactories”withinadegenera
8、teepithelialcellfromdamagedcolonicmucosaofcowwithwinterdysentery.Bar=200nm.566BriefCommunications:;-4,Fig.6.Extensiveimmunoperoxidasereactivitywi
