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1、VIROLOGY116,310-321(1981)RNAandPolypeptideHomologyamongMurineCoronavirusesSTEVECHELEY,VINCENTL.MORRIS,MARGARETJ.CUPPLES,ANDROBERTANDERSON’DepurtWofMirrobidosyandImmww~,UniversityofWe&s-nOntario,LondonN6A5ClCanadaReceivedMay8,1981;acceptedAugust10,1981Usinga=Pc
2、omplementaryDNA(cDNA)preparedagainsttheA59nucleocapsidproteinmessengerRNA,wehaveinvestigatedtheextentofhomologybetweenA59andfourotherstrainsofmurinehepatitisvirus(MHV).AnalysisbyhybridizationkineticsoftheannealingbetweenA59[S2P]cDNAandinfectedcellRNAfromtheoth
3、erfourMHVstrainsdemonstrated7040%homology.Bygeltransferanalysis,theA59[S2P]eDNAwasabletodetectsubgenomic-sizevirus-specificRNAsincellsinfectedwithallofthefiveMHVstrains.AsimilarpatternofsevenviralRNAswasdetectedincellsinfectedwithA59,MHV-1,MHV-3,andJHM.Incontr
4、ast,cellsinfectedwithMHV-Scontainedsevenvirus-specificRNAs,ofwhichonlythetwosmallestspeciescomigratedwithRNAsfromtheotherfourstrains.TheresultssuggestthataspreviouslyshownwithA59(S.Cheley,R.Anderson,M.J.Cupples,E.C.M.LeeChan,andV.L.Morris(1981)Virdogy,112,596-
5、604),allMHVstrainstestedencodeanestedsetofsubgenomicRNAs.Analysisof[?l]methionine-labeledviralproteinsbySDS-polyacrylamidegelelectrophoresisre-vealedthateachstrainofMHVspecifiedfourmajorviralpolypeptideswithapparentmolecularweightsverysimilartothosepreviouslyr
6、eportedfortheE2,N,El,andPElpolypeptidesofA59.ThestrongdegreeofinterstrainhomologyamongthefiveMHVstrainsinvestigatedwasconfirmedbycomparativechymotrypticpeptidemappingoftheviralNproteins.Amajorityofthechymotrypticpeptidesfromeachofthe[35S$nethionine-labeledNpro
7、teinswasfoundtocoelutebyhigh-performanceliquidchromatography.Moreover,thistechniqueofpeptidemappingindicatedaparticularlystrongrelatednessbetweenMHV-1andMHV-SandamongMHV3,JHM,andA59.INTRODUCTIONMHVstrains,showsonlyamoderatepro-pensitytowardvisceraltissue,inste
8、ad,Coronavirusesincludeanumberofviralproducingaselectiveinfectionofthener-pathogenswhichareresponsibleforadi-voussystem(BaileyetaL,1949;Cheeverversespectrumofpathologicalconditions
