冠狀病毒Coronavirusl論文-1992 Bovine coronavirus uses N-acetyl-9-O-acetylneuraminic acid as a receptor determinant to initiate the infection of c.pdf

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1、JournalofGeneralVirology(1992),73,901-906.PrintedinGreatBritain901BovinecoronavirususesN-acetyl-9-O-acetylneuraminicacidasareceptordeterminanttoinitiatetheinfectionofculturedcellsBeateSchultzeandGeorgHerder*InstitutfarVirologie,Philipps-Universiti~tMarburg,Robert-Koch-Stras

2、se17,3550Marburg,GermanyTheimportanceofN-acetyl-9-O-acetylneuraminicacidasialo-MDCKicellswithNeu5,9Ac2.Transferof(Neu5,9Ac2)asareceptordeterminantforbovinesialicacidlackinga9-O-acetylgroupwasineffectiveincoronavirus(BCV)onculturedcellswasanalysed.thisrespect.Theseresultsdem

3、onstratethat9-0-PretreatmentofMDCKI(MadinDarbycanineacetylatedsialicacidisusedasareceptordeterminantkidney)cellswithneuraminidaseoracetylesterasebyBCVtoinfectculturedcells.ThepossibilityisrenderedthecellsresistanttoinfectionbyBCV.ThediscussedthattheinitiationofaBCVinfection

4、involvesreceptorsonahuman(CaCo-2)andaporcine(LLC-therecognitionofdifferenttypesofreceptors,afirstPK1)epithelialcelllinewerealsofoundtobesensitivereceptorforprimaryattachmentandasecondreceptortoneuraminidasetreatment.Thesusceptibilitytotomediatethefusionbetweentheviralenvelo

5、peandinfectionbyBCVwasrestoredafterresialylationofthecellularmembrane.Introductionerythrocytes(Yokomorietal.,1989),whereasBCVisapotenthaemagglutinatingagent(Satoetal.,1977).Bovinecoronavirus(BCV)isanenteropathogenicThecrucialstepintheelucidationoftheerythrocytecoronavirusca

6、usingseverediarrhoeainnewborncalvesreceptorsforBCVwasthefindingthatBCVand(Siddelletal.,1983).ThemechanismsbywhichBCVinfluenzaCvirushaveareceptor-destroyingenzymeininfectscellsandcausesdiseasearenotwellcharacter-common.InfluenzaCvirusinactivatesitsownreceptorsized,norarethef

7、actorsknownwhichdeterminethehostbyavirus-encodedacetylesterase,whichreleasesO-andtissuespecificity.ThecellularreceptorwhichisaacetylresiduesfrompositionC9ofN-acetyl-9-O-crucialdeterminantofthetropismofseveralviruses,isacetylneuraminicacid(Neu5,9Ac2)(Herderetal.,notknowninth

8、ecaseofBCV.1985b).ThesameenzymeisalsopresentonthesurfaceofTheonlyreceptorforcorona