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1、Vrology_ArchivesArchVirol(1994)134:421-426?Springer-Verlag1994PrintedinAustriaComparisonoftheSgenesandthebiologicalpropertiesofrespiratoryandenteropathogenicbovinecoronavirusesBriefReportX.Zhang1,W.Herbst2,K.G.Kousoulas1,andJ.Storz11DepartmentofVeterinaryMicrobiologyandParasitology,Schoo
2、lofVeterinaryMedicine,LouisianaStateUniversity,BatonRouge,LouisianaU.S.A.2Institutf/JrHygieneundInfektionskrankheitenderTiere,Justus-LiebigUniversit~itGiessen,Giessen,FederalRepublicofGermanyAcceptedSeptember7,1993Summary.ThenucleotidesequenceoftheSgeneofthebovinerespiratorycoronavirus(B
3、RCV)strainG95,whichwasisolatedfromnasalswabsofacalfsufferingfromrespiratorydisorders,wasdeterminedandcomparedwiththeSgeneoftheenteropathogenicbovinecoronavirus(BECV)strainLY138.Sequenceanalysisrevealed98.7%nucleotideand98.3%deducedaminoacididentitiesbetweentheSgenesofBRCV-G95andBECV-LY13
4、8withoutanydeletionsorinsertions.Nucleotidesubstitutionsweredistributedrandomlythroughoutthegene.FivemonoclonalantibodiesspecificfortheSproteindistinguishedBRCV-G95fromBECV-L9,butfailedtodifferentiateitfromBECV-LY138inWesternblotsunderdenaturedandnativeconditions.BRCV-G95inducedcytopathi
5、cchangesincellculturesthatweresimilartoBECV-LY138butdifferentfromBECV-L9.TheseresultssuggestthatstrainBRCV-G95ismorecloselyrelatedtothevirulentstrainBECV-LY138thantotheavirulent,cellculture-adaptedstrainBECV-L9.Bovinecoronavirus(BCV)causessevereenteritisofnewborncalves[3,11,12],anditisal
6、sorecognizedasanetiologicalfactorofrespiratorydiseaseofcalves[5,6,9,13,15].Comparativestudiesrevealedthatcoronavirusisolatesfromtheintestinalorrespiratorytractsofcalvesbothreplicatedinintestinalandupperrespiratorycellsofgnotobioticcalves[17,18].Serologicaltestsfailedtodistinguishbetweenb
7、ovinerespiratorycoronavirus(BRCV)andentero-pathogenicbovinecoronavirus(BECV).Aporcinerespiratorycoronavirus(PRCV)hadadeletionintheSgenewhencomparedwiththetransmissible422X.Zhangetal.gastroenteritisvirus.Thisdeletionwasconsideredmostlikelyresponsibleforthedifferencesintiss
