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1、S198Abstracts/NeuroscienceResearch58S(2007)S1–S244P3-c16TheresponseofmicrogliaandastrocytestotheP3-c19Astrocyte-mediatedregulationofsynapticglutamateinfectedcorticalneuronsinducedbyacoronavirusreleaseintheNTSWanZhuBai1,LiYanChao1,HiranoN.2,TohyamaT.3,TaikoImura,NamiHasegawa,MikaWako,FusaoKato1Labo
2、ratoryofNeurophysiology,DepartmentofNeuroscience,JikeiHashikawaT.1BrainScienceInstitute,RIKEN,Japan;2DepartmentofUniversitySchoolofMedicine,Tokyo,JapanVeterinaryMicrobiology,IwateUniversity,Japan;3CenterforEM&Bio-ImagingResearch,IwateMedicalUniversity,JapanInthenucleusofthesolitarytract(NTS),spati
3、allydelimitedactivationofpresynapticP2Xreceptorswithlaser-basedphotoactivationofcagedATPinthebrainslicesfacilitatesglutamaterelease,affectingtheexcitabil-Followinginoculationofacoronavirus(strain67N)intotherathindfoot-ityofthepostysynapticneurons(ShigetomiandKato,2004;Imuraetal.,pad,thetimecourseo
4、ftheneuronalinfectionandglialactivationwere2006).WeexaminedthehypothesisthatATPofastroglialorigincouldacti-immunohistochemicallyexaminedintheprimarymotorcortex(M1).ThevatethesepresynapticP2XreceptorsandaffectsynaptictransmissioninactivatedmicrogliaandastrocytesassociatedwithinfectedneuronsweretheN
5、TS.Electron-microscopicanalysesrevealedthat99%ofpresynaptic?rstdetectedinthelayer5ofM1contralaterallytotheinjectionsideonterminalsshowedpartialorfullcontactwithastrocyte-likeprocesses.day4post-inoculation(p.i.),andshowedwithenlargedcellbodiesandTwolinesofevidence,ascon?rmedintheratbrainstemsliceus
6、ingthickenedprocesses.Furthermore,activatedmicrogliamigratedtoandpatch-clamprecordingofsynapticcurrents,that(1)activationofP2Y1gatheredaroundtheinfectedneurons.Onday5p.i.,activatedmicrogliareceptors,thataremostlyexpressedinastrocytes,increasedmEPSCchangedintoamoeboidformandtightlyensheathedtheinfe
7、ctedneu-frequencyand(2)atreatmentwith?uoroacetatedecreasedmEPSCfre-rons.Additionally,numerousOX42-immunoreactivecellsinroundshapequencysupportthenotionthatthesepresynapticP2Xreceptorsmightappearedontheinfectedneu
