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1、南方農(nóng)業(yè)學(xué)報(bào)JournalofSouthernAgriculture2015,46(3):480--485ISSN2095—1191;CODENNNXAABhttp://www.nfnyxb.cnD01:10.3969,j:issn.2095-1191.2015.3.480響應(yīng)面法優(yōu)化竹蓀菌托多糖的提取工藝吳雪艷,羅鵬+,葉建方,肖佳艷,張?jiān)ィ″|,張吉梅,劉繼中(貴陽醫(yī)學(xué)院公共衛(wèi)生學(xué)院,貴陽550004)摘要:【目的】優(yōu)化竹蓀菌托多糖的提取工藝,為其開發(fā)利用提供技術(shù)支持。【方法】以竹蓀菌托為原料
2、,通過單因素試驗(yàn)分析提取溫度、提取時(shí)間、液料比、提取次數(shù)對竹蓀菌托多糖提取率的影響,并在此基礎(chǔ)上采用Box.Benhnken響應(yīng)面法建立以竹蓀菌托多糖提取率為響應(yīng)值的二次回歸數(shù)學(xué)模型?!窘Y(jié)果】通過響應(yīng)面分析建立竹蓀菌托多糖提取回歸方程為:Y=12.96+0.29A+O.13B+0.17C一0.13AB一0.039AC一0.014BC一0.34A2-0.2782-0.34C2(A為提取溫度,B為提取時(shí)間,C為液料比,Y為竹蓀菌托多糖提取率,Rk0.9551),該模型擬合度好;并確定竹蓀菌托多糖提取的
3、影響因素順序?yàn)椋禾崛囟?液料tt>提取時(shí)間,其中提取溫度和液料比對竹蓀菌托多糖提取率有極顯著影響(P<0.01),提取時(shí)間有顯著影響(P<0.05),但雙因素交互作用對竹蓀菌托多糖提取率無顯著影mj(e>0.05);竹蓀菌托多糖最佳提取工藝條件為:在提取溫度82℃、液料£L62:lmL/g的條件下提取3。Ih、提取1次,竹蓀菌托多糖提取率為13.016%,與模型預(yù)測理論值13.040%接近?!窘Y(jié)論】采用響應(yīng)面法優(yōu)化竹蓀菌托多糖提取工藝具有口I行性,可用于實(shí)際生產(chǎn)。關(guān)鍵詞:竹蓀菌托;多糖;響應(yīng)面法
4、;工藝優(yōu)化中圖分類號:TQ914.3文獻(xiàn)標(biāo)志碼:A文章編號:2095—1191(2015)03—0480—06OptimizationofextractiontechniqueofpolysaccharidefromvolvaofDictyophoraindusiatabyresponsesurfacemethodologyWUXue-yan,LUOPeng+,YEJian-fang,XIAOJia-yan,ZHANGYu,DINGKai,ZHANGJi-mei,LIUJi-zhong(Schoo
5、lofPublicHealth,GuiyangMedicalUniversity,Guiyang550004,China)Abstract:【Objective】卟eextractionparametersofpolysaccharideinvolvaofDictyophoraindusiatawereoptimizedtoprovidetechnicalsupportforitsfurtherdevelopment.【Method】VolvaofD/ctyophoraindusiatawasus
6、edasmateri—a1.Single—factorexperimentsandresponsesurfacemethodologywereusedtoselectappropriatelevelsonextractiontern—perature.extractiontime.1iquid—to—materialratioandextractiontimes.Aquadraticregressionmodeldescribingtheel-fectsofextractionconditions
7、ontheextractionrateofDolvsaccharidewasdevelopedbyBox—Behnkenexperimentalde—sign.[Result]ThequadraticmuhinomialregressionequationofpolysaccharideextractioninvolvaofDietyophoraindusi—atawasestablishedasY=12.96+0.29A+0.13B+0.17C一0.13AB一0.039AC一0.014BC一0.
8、34A2—0.2782—0.34C2(A:extrac.tiontemperature,B:extractiontime,C:liquid—materialratio,Y:extractionrateofpolysaecharide,R2-0.9551),andthemodelfittedwellwiththeexperimentaldata.Theinfluencingorderofparametersontheextractionratewasextractiontempera