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1、#30#中國實(shí)用醫(yī)藥2010年4月第5卷第12期ChinaPracMed,Apr2010,Vo.l5,No.12慢性乙型肝炎患者血清IL-12、IL-6的水平變化和CD28表達(dá)的研究李蕾高志星季萬勝張小茜張紅梅=摘要>目的探討IL-12、IL-6和淋巴細(xì)胞CD28分子在慢性乙型肝炎發(fā)病中的作機(jī)制。方法嚴(yán)格按照病例入選標(biāo)準(zhǔn)選取50例患者和20例健康志愿者,每例空腹抽取外周靜脈血5m,l3000rpm離心+10min,吸取上層血清110m,l放置-70e冰凍保存?zhèn)錂z,同時(shí)通過流式細(xì)胞儀檢測所有患者外周血CD28淋巴細(xì)胞所占
2、比例。采用雙抗體夾心ABC-ELISA法檢測患者外周靜脈血血清中細(xì)胞因子IL-12、IL-6的水平,比較慢性乙型肝炎患者與健康對照組及急性乙型肝炎之間Th1和Th2細(xì)胞因子表達(dá)的差異。結(jié)果HBeAg(-)慢性乙型肝炎患者IL-12、IL-6水平顯著高于健康對照組(P<0101),HBeAg(-)慢性乙型+肝炎患者CD28T淋巴細(xì)胞所占比例升高,顯著高于健康對照組(P<0101),急性乙型肝炎組水平最高,+HBeAg(-)慢性乙型肝炎患者CD28T淋巴細(xì)胞水平低于HBeAg(+)慢性乙型肝炎患者,差異有統(tǒng)計(jì)學(xué)意義(P<0
3、101)。結(jié)論慢性乙型肝炎患者Thl型細(xì)胞因子表達(dá)強(qiáng)弱與HBV感染肝臟的炎癥活動密切相關(guān);Th2細(xì)胞因子表達(dá)增強(qiáng),與HBV的持續(xù)感染、免疫耐受形成有關(guān);CD28分子在乙型肝炎病毒的清除過程中起重要作用。=關(guān)鍵詞>慢性乙型肝炎;白細(xì)胞介素-12;白細(xì)胞介素-6;CD28ThestudyofthelevelofIL-12,IL-6inserumandtheexpressionofCD28onlymphocyteinHBeAg(-)CHBpatientsLILei,GAOZhi-xing,JIWan-sheng,etal.D
4、epartmentInternalMedicineofWeifangMedicalCollege,ShandongWeifang261042,China=Abstract>ObjectiveTodiscussthemechnismofThelpercellsandtheircytokinesinpatientswithhepatiticBviruseantigene(HBeAg)negetivechronichepatitisB(CHB).MethodsWechoosedfortypatientsandtenhealt
5、hyvolunteersstrictly.Wetook5millilitresperipheralbloodfromeachpersonandhavethreemillil-itresofthemcentrifugedfortenminutesatthespeedof3000rpmandtookonemillilitreserumtotherefrigeratorwhichthetemperatureis70ebelowzeroforfutureassay.WedetectedtheproductionofIL-12a
6、ndIL-6inallthesespecmiensbyavidin-biotincomplex-enzymelinkedmimunosorbentassay(ABC-ELISA).ResultsThere-sultsofHBeAgnegetivegroupshowedthatIL-12,IL-6wereallsignificantlyupperadjusted(comparedtothehealthycontrolgroup:P<0105).TheresultofAHBshowedthatIL-6wassignific
7、antlydownadjusted(compared+tothehealthycontrolgroup:P=0102).TheproportionofCD28TlymphocyteinHBeAgnegetiveCHBgroupwaslargerthanthatofthehealthycontrolgroup,butitwassmallerthanthatofHBeAgpositiveCHBgroup(P<+0105).TheproportionofCD28TlymphocyteintheAHBgroupwaslarge
8、stinthefourgroups.ConclusionThedisbalanceofcellmimunityandhumoralmimunityisexistedintheHBeAgnegetiveCHBpatients.TheamountsofcytokinsfromTh1cellshavecloserelationshipw
