資源描述：
《藤本豆根結(jié)線蟲病的病原鑒定及其侵染特性的研究-論文.pdf》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、熱帶作物學(xué)報2012，33(2)：346—352ChineseJournalofTropicalCrops藤本豆根結(jié)線蟲病的病原鑒定及其侵染特性的研究劉國坤，肖順，張紹升，張敦富，吳秋月福建農(nóng)林大學(xué)植物保護(hù)學(xué)院，福建福州，35002摘要在福建省發(fā)現(xiàn)豆科新品種多年生藤本豆(Lablabsp．)發(fā)生嚴(yán)重的根結(jié)線蟲病，根部可產(chǎn)生巨大根結(jié)，根結(jié)內(nèi)組織內(nèi)可埋生數(shù)以百計的雌蟲，卵囊產(chǎn)于根結(jié)內(nèi)。通過形態(tài)特征及rDNA—IrS區(qū)序列擴(kuò)增比對，藤本豆根結(jié)線蟲鑒定為南方根結(jié)線蟲(MeloidogyneincognitⅡ)。多年生藤本豆受根結(jié)線蟲為害為首次記述，也是南方根結(jié)線蟲新寄主。接種
2、實(shí)驗(yàn)結(jié)果表明，2齡幼蟲主要侵染藤本豆根尖，也可侵染根其他部位，雌蟲與卵囊埋生于根結(jié)內(nèi)，卵可直接孵化并在根內(nèi)直接侵染發(fā)育，根結(jié)大小發(fā)展快，根結(jié)堅硬，表面組織不易腐解，雄蟲只能在后期根結(jié)腐敗組織處發(fā)現(xiàn)。在環(huán)境溫度(2436oC)，生活史最短為24d。田間重要雜草空心蓮子草(Afzernantheraphiloxeroides)根結(jié)線蟲病發(fā)生普遍，形態(tài)特征鑒定為南方根結(jié)線蟲，田間調(diào)查及交叉接種實(shí)驗(yàn)表明其可能為田問藤本豆根結(jié)線蟲病的主要侵染來源關(guān)鍵詞藤本豆；南方根結(jié)線蟲；鑒定；侵染特性；空心蓮子草中圖分類號s435．29文獻(xiàn)標(biāo)識碼AIdentificationofRoot-K
3、notNematodeSpeciesParasitizingLablabsp．a(chǎn)ndItSInfectionCharacteristicsLIUGuokun，XIAOShun，ZHANGShaosheng，ZHANGDunfu，WuQiuyueCollegeofPlantprotection,PujianAgricultureandForestryUniversity，~ujian，F(xiàn)uzhou350002，ChinaAbstractSevereoccurrenceofroot—knotdiseaseofLablabsp．wasfoundinFujianProvinc
4、e，China．ThegallsonrootsofLablabsp．tendtobelargecontaininghundredsoffemales．Eggmassesweredepositedwithinthegal1．BasedonmorphologicalcharacteristicsandregionsofrRNAITSsequences，theroot-knotnematodeonLabZsp．identifiedasMeloidogynencognita．ThisiSthefirstreportthatLablabsD．wasinfestedbyroot—
5、knotnematodeanditwasthenewrecordhostforincognita．Theresultsofinoculationshowedthatsecond—stageiuvenilesofroot—knotnematodeusuallypenetratedtheroot-tipofLablabsp．，butalsopenetratedtheothersitesofroots．Eggmassesproducedbymaturedfemaleswereentirelyembeddedintheroot．Afterhatchinginsidethero
6、ot．secondaryinfectionsofthesecondiuvenilesmaytakeplaceintheroot—knot．Theroot—knotinduecdbyroot—knotnematodesdevelopedrapidlytolargegalls．rhemalecouldbefoundonlyindecayingrootatlategrowthstageofroot—knot．Theshortestlifecyclewascompletedin24daysatanambienttemperaturesof24～36oC．Commonoccur
7、renceofroot—knotdiseaseofAZternantheraphiloxeroideswasalSOfoundinthefieldofLablabsD．Theroot—knotnematodeonA．philoxeroideswasidentifiedasincognitbaseonmorphologicalcharacteristics．Thefieldinvestigationandcross—inoculationexperimentsshowedthattherootknotnematodeofA．philoxeroidesw
