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1、HPLC法同時(shí)測定開胸順氣丸中橙皮苷、和厚樸酚與厚樸酚的含量劉宏明,楊福偉。(1.山東大學(xué)藥學(xué)院,濟(jì)南250012;2.淄博市藥品檢驗(yàn)所,山東淄博255086;3.淄博職業(yè)學(xué)院,山東淄博255314)中圖分類號(hào)R917文獻(xiàn)標(biāo)志碼A文章編號(hào)1001.0408(2012)40.3825.03DO110.6039/j.issn.1001-0408.2012.40.30摘要目的:建立同時(shí)測定開胸順氣丸中橙皮苷、和厚樸酚與厚樸酚含量的方法。方法:采用高效液相色譜法。色譜柱為Agi.1entZORBAXSB.C。柱,流動(dòng)相為甲醇(A).5%冰醋酸溶液(B)(梯度洗脫),流速為1.0mL·min_。,柱
2、溫為40℃,進(jìn)樣量為10,檢測波長0~20rain為284nm、20~65min為294fin1。結(jié)果:橙皮苷進(jìn)樣量在0.1717~4.292g范圍內(nèi)(r=0.9998)、和厚樸酚進(jìn)樣量在O.04004~1.001g范圍內(nèi)(r=O.9994)、厚樸酚進(jìn)樣量在O.09280~4.640g范圍內(nèi)(,=0.9998)與各自峰面積積分值均呈良好的線性關(guān)系;平均回收率分別為99.88%、99.34%、99.34%,RSD分別為0.67%、1.1O%、0.86%(=6)。結(jié)論:本法簡便、準(zhǔn)確、重復(fù)性好、精密度高,可更好地控制開胸順氣丸的質(zhì)量。關(guān)鍵詞開胸順氣丸;橙皮苷;和厚樸酚;厚樸酚;高效液相色譜法;
3、含量測定SimultaneousContentDeterminationofHesperidin,HonokiolandMagnoloinKaixiongShunqiPillsbyHPLCLILYHong—ming(SchoolofPharmacy,ShandongUniversity,Jinan250012,China)LIUHong—ming(ZiboInstituteforDrugControl,ShandongZibo255086,China)YANGFu—wei(ZiboVocationalInstitute,ShandongZibo255314,China)ABSTRACT0B
4、JECTIVE:Toestablishthemethodforthecontentdeterminationofhesperidin,honokiolandmagnololinKaix.iongshunqipills.METHODS:HPLCmethodwasadopted.TheseparationwereachievedonanAgilentZORBAXSB.Cl8columnwithmethanol(A).5%aceticacidsolution(B)asmobilephase(gradientelution)attheflowrateof1.0mL·min一1.Thecolumnt
5、emperaturewas40℃andthedetectionwavelengthswere284nmat0~20minand294nmat20~65min.Theectionvolumewas10.RESULTS:Thelinearrangewas0.1717~4.292gforhesperidin(,=0.9998),0.04004~1.o01ugforhonokiol(r=0.9994)and0.0928O~4.640Bgformagnolo1(r:0.9998);theaveragerecoverieswere99.88%(RSD:0.67%,=6),99.34%(RSD=1.1%
6、。”=6)and99.34%(RSD=0.86%,/,/:6),respectively.CONCLUS10N:Themethodissimple,accurate,repro.ducibleandprecise.a(chǎn)nditissuitableforthe0ualitycontrolofKaixiongshunqipills.KEYWoRDSKaixiongshunqipills;Hesperidin;Honokiol;Magnolol;HPLC;Contentdetermination表2樣品含量測定結(jié)果(=2)8O的含量檢測。此外,該方法也適合在623.50nm波長處測定Tab2Res
7、ultsofcontentdeterminationofsamples(n=2)其他陰性對(duì)照無干擾的含聚山梨酯80的中藥注射劑中該成分的含量。參考文獻(xiàn)[i】王慶利,彭?。聹?0的安全性研究進(jìn)展[J].毒理學(xué)雜志,2006,20(4):262.[2】DaherCF,BaroodyGM,HowlandRJ.Effectofasurfac-tant,Tween80,ontheformationandsecretionofch