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1、外科雜志2014年5月第31卷第5M‘dv2O14．Vo1．31．No．5·1045··實(shí)驗(yàn)研究·低功率超聲增強(qiáng)順鉑對食管鱗癌細(xì)胞的抗腫瘤作用黨麗峰楊洋劉東雷吳愷溫豐標(biāo)趙松【摘要】目的觀察低功率超聲在增強(qiáng)Nef對食管鱗癌細(xì)胞抗腫瘤作用中的影響。方法以食管鱗癌EC9706細(xì)胞為實(shí)驗(yàn)對象，分別利用單純低功率超聲(0．5W／cm和1．0w／cm。)、單純順鉑(1Ixmol／L)、先超聲處理再加入順鉑(超聲+順鉑)和先加入順鉑再應(yīng)用超聲(順鉑+超聲)。4種方式干預(yù)細(xì)胞的生長，細(xì)胞計(jì)數(shù)試劑盒(CCK一8)法檢測細(xì)胞活力，顯微鏡下定
2、性觀察熒光染色，流式細(xì)胞術(shù)定量檢測細(xì)胞凋亡。結(jié)果超聲功率分別為0．5w／cm。和1．0W／cm時，CCKI8法檢測細(xì)胞活力，與對照組(3．78％、3．97％)比較，低功率超聲(14．36％、15．43％)明顯降低了腫瘤細(xì)胞活力(P<0．05)。超聲功率為0．5w／cm時，順鉑+超聲組(cisPt+us)細(xì)胞凋亡率為(67．23±4．38)％，明顯高于超聲+順鉑組(US+cisPt)的(75．38±3．98)％(P<0．05)。結(jié)論低功率超聲可以提高腫瘤細(xì)胞對化療藥物的敏感性，抗腫瘤藥物和超聲序貫干預(yù)的先后順序，有助于提高
3、腫瘤殺傷療效。【關(guān)鍵詞】低功率超聲；順鉑；食管鱗癌；脫噬作用Enhancinganticancerefectofcisplatinonhumanesophagealsquamouscellcarcinomainvitrodur-inglow-frequencyultrasoundexposureDangLifeng，YangYang，LiuDonglei，WuKai，耽nFeng-biao，ZhaoSong．MedicalExaminationCenter，危eFirstAffiliatedHospitalofZheng
4、zhouUniversity．Zhengzhou450052，ChinaCorrespondingauthor：ZhaoSong，EmaiZ：zhaosong@ZZU．edu．cn【Abstract】0bjectiveToexploretheantitumoreffectofcisplatincombinedwithlow．frequencyul—trasoundonhumanesophagealsquamouseellcarcinomainvitro．MethodsFourmodesoftreatmentwereuse
5、d：exposuretolow—powerultrasound，applicationofcisplatin，exposuretoultrasoundfollowedbycispla—tin．a(chǎn)ndpresenceofcisplatinfollowedbyexposuretoapplicationultrasound．Ultrasoundwasusedatinten．sitiesof0．5W／cmand1．0W／emforl0min．a(chǎn)ndcisplatinwasappliedatconcentrationof1p~mo
6、L／LpercellsuspensiontreatedinEC9706cells．respectively．Theresultsofeachexperimentaltreatmentwereassessedbycellcountingkit一8(CCK一8)assay．FlowcytometrywasperformedtoexaminetheapoptosisrateofcellstreatedwithAnnexinV—fluoresceineisothiocyanate(FITC)／proliferationindex
7、(PI)doublestaining．ResultsThecombinedeffeetofultrasoundandcisplatinwasmoreeffeetivethanthatofultra—soundorcisplatinalone．Italsoappearedthattheorderofapplicationplayedarole，withthecisplatin—ul—trasoundtreatmentloweringcellviabilitymorethantheultrasound—eisplatintr
8、eatment．ConclusionLow．frequencyultrasoundcanpromotethecytotoxicityofcisplatinandtheorderofapplicationplayedaroleinenhancingthesensitivityofchemotherapydrugsont
