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1、作物學(xué)報ACTAAGRONOMICASINICA2013，39(4)：609—616http：／／www．chinacrops．org／zwxb／ISSN0496—3490；CODENTSHPA9E-mail：xbzw@chinajourna1．net．cnD0I：10．3724／SP．J．1006．2013．00609大豆粒形性狀QTL的精細定位牛遠謝芳騰布素紅謝尚潛韓世鳳耿青春劉兵章元明南京農(nóng)業(yè)大學(xué)作物遺傳與種質(zhì)創(chuàng)新國家重點實驗室，江蘇南京210095摘要：在溧水中子黃豆×南農(nóng)493—1衍生的504個F2：6家系中選擇Satt331-Satt592目標(biāo)區(qū)
2、間7個雜合單株和168個重組單株，衍生成356株RHL．F2個體(群體I)和168個重組體家系(群體II)。群體II來自142個F2：6家系，若每個F2：6家系只保留1個重組家系則構(gòu)成群體III。采用lasso和復(fù)合區(qū)間作圖(CIM)法檢測3個群體粒形性狀2種指標(biāo)的QTL。結(jié)果表明，lasso法檢測到的粒長關(guān)聯(lián)標(biāo)記是O19和s21／Satt331，而CIM檢測到的QTL區(qū)間是S21-S22和023-019；lasso法檢測到的粒寬關(guān)聯(lián)標(biāo)記是O19／021，而CIM檢測到的QTL區(qū)間是023～O19／O19-O21；長寬比與s21～$22關(guān)聯(lián)是由于粒長QTL引
3、起的，與023～O19／O19～021關(guān)聯(lián)是由于粒長和粒寬QTL引起的。將原Satt331-Satt592目標(biāo)區(qū)間的粒長QTL剖分為與標(biāo)記$21～$22和023～O19／O19-021關(guān)聯(lián)的2個多效性QTL。根據(jù)大豆基因注釋數(shù)據(jù)庫，Glymal0g35240和Glymal0g34980可能是控制粒形性狀發(fā)育的候選基因。關(guān)鍵詞：大豆：粒形：數(shù)量性狀基因座；精細定位；候選基因FineMappingofQuantitativeTraitLociforSeedShapeTraitsinSoybeanNIUYuan，XIEFang—Teng，BUSu—Hong，XIE
4、Shang-Qian，HANShi—Feng，GENGQing—Chun，LIUBing，andZHANGYuan—MingStateKeyLaboratoryofCropGeneticsandGermplasmEnhancement，NanjingAgriculturalUniversity,Nanjing210095，ChinaAbstract：BasedonthetargetintervalregionofSSRmarkersSaR331-Satt592．weconstructed356RHL．F々individuals(popula．tionI)an
5、d168recombinantfamilies(populationII)derivedfrom504F26familiesofthedirectandreciprocalcrossesofLishuizhongzihuangwithNannong493—1tofinemapquantitativetraitsloci(QTLs)forseedlength(SL)，width(SW)，andlength-to—width(SLw)ratioinsoybeanusingcompositeintervalmapping(CIM)andlassoapproache
6、s．Amongthe168recombi—nantfamilies，onlyonerecombinantindividualwasselectedfromasameF，．familysothat142recombinantfamilieswerecon．structedaspopulationIII．ResponsevariableforQTLanalysiswasasorigina1observation．a(chǎn)ndthevaluecorrectedbytheassociatedmarkerinformation．Asaresult．markersassoci
7、atedwithSLwereO19andS21／Satt331fromlassomethodandS21-$22andO23—o19fromCIMmethod；markerassociatedwithSWwasO19／O21fromlassomethodandO23—019／Ol9一O21fromCIMmethod；andtheOTLassociatedwithmarkers$21-$22forSLWwasderivedfromQTLforSL，andtheQTLwithmarkers023—O19／O19—021forSLWwasderivedfromQT
8、LsforSLandSWThisindicatest
