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1、第23卷第2期中國組織化學(xué)與細(xì)胞化學(xué)雜志V01．23．No．22014年4月CHINESEJOURNALOFHISTOCHEMISTRYANDCYTOCHEMISTRYApril．2014當(dāng)歸多糖對輻射損傷小鼠骨髓單個核細(xì)胞凋亡的影響何曉莉陳鳳鳴吳宏肖倩(重慶醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院組織胚胎學(xué)教研室，干細(xì)胞與組織工程研究室，重慶400016)[摘要]目的探討當(dāng)歸多糖(Angelicapolysaccharides，APS)對輻射損傷小鼠骨髓單個核細(xì)胞(Bonemarrownlononuclearcell，BMNC)凋亡的影響，旨在闡明APS對輻射性造血損傷保護作用
2、的分子機制。方法結(jié)果結(jié)論方法CBL／6小鼠隨機分為1O組，正常組不作任何處理，其余9組經(jīng)x射線(4．0Gy)照射后分別給予不同劑量APS或生理鹽水，并于照射后第1、3、7d處死小鼠，流式細(xì)胞術(shù)檢測BMNC凋亡率、Bcl一2表達(dá)率，WesternBlot法(wB)檢測P53蛋白、Caspase一3蛋白的表達(dá)。結(jié)果與正常組相比，生理鹽水組BMNC凋亡率、P53蛋白、Caspase-3激活片段(12+17KD)的表達(dá)升高，Bcl一2及Caspase一3全長片段(35KD)的表達(dá)降低。與生理鹽水組相比，2mg／kgAPS組和8mg／kgAPS組均能降低凋亡率，P53
3、蛋白及Caspas~3激活片段的表達(dá)，升高Bcl一2和Caspase一3全長片段的表達(dá)。結(jié)論APS對輻射引起的細(xì)胞凋亡具有一定的保護作用[關(guān)鍵詞]當(dāng)歸多糖；輻射損傷；細(xì)胞凋亡；骨髓單個核細(xì)胞；Caspase一3(中圖分類號]Rl46(文獻標(biāo)識碼]AD0I：10．3870／zgzzhx．2014．O2．O13EffectsofangelicapOlysaccharideonapoptosisofbonemarrowmononuclearcellsinmicewithionizingradiationinjuryHeXiaoli，ChenFengming，WuH
4、ong，XiaoQian(DepartmentofHistologyandEmbryology，LaboratoryofStemCellandTissueEngineering，F(xiàn)acultyofBasicMedicalSciences，ChongqingMedicalUniversity，Chongqing400016，China)(Abstract3ObjectiveTostudytheeffectsofangelicapolysaccharide(APS)onthecellapoptosisofbonemarrowmononuelearcells(BM
5、NC)inmicewithionizingradiationiniury，soastoilluminatethemo—lecularmechanismofprotectiveeffectsofAPSagainstradioactiveinjuredhaematogenesis．MethodsC57BL／6miceweredividedinto10groupsatrandom．Thenormalgroupwasnotradiated．Theother9groupswereradiatedbyX—ray(4．0Gy)，andthengivendifferentd
6、osagesofAPSornormalsaline．Themicewerekilledat1，3，7dafterradiation．TheapoptosisrateofBMNCandtheexpressionofBel一2weredetectedbyflowcytometry(FCM)，theexpressionCaspase一3andp53weredetectedbyWesternBlot(WB)．ResultsComparedwiththoseofthenormalgroup，theapoptosisrateofBMNC。theexpressionofP
7、53andtheactivatedfragmentofCaspase一3increasedsignificantly。whiletheexpressionofBcl一2andthefullfragmentofCaspase一3obviouslydecreasedinNSgroups．ComparedwiththoseoftheNSgroups，theapoptosisrateofBMNC，theexpressionofP53andtheactivatedfragmentofCaspase一3decreased，whiletheexpressionofBcl一
8、2andthefullfragmentofCaspa
