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《沒(méi)藥甾酮對(duì)膽管癌 RBE 細(xì)胞移植瘤生長(zhǎng)和 miRNA表達(dá)譜的影響.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、．640．安徽醫(yī)藥AnhuiMedicalandPharmaceuticalJournal2015Apr；19(4)沒(méi)藥甾酮對(duì)膽管癌RBE細(xì)胞移植瘤生長(zhǎng)和miRNA表達(dá)譜的影響仲飛，童鑄廷，范璐璐，姚夢(mèng)群，孫國(guó)平(安徽醫(yī)科大學(xué)第一附屬醫(yī)院，安徽合肥230022)摘要：目的研究沒(méi)藥甾酮對(duì)膽管癌RBE細(xì)胞株移植瘤生長(zhǎng)和miRNA表達(dá)的影響。方法雌性SD大鼠建立膽管癌RBE細(xì)胞株移植瘤模型，隨機(jī)分為對(duì)照組和沒(méi)藥甾酮高、低劑量實(shí)驗(yàn)組；高、低劑量實(shí)驗(yàn)組大鼠分別予40、20mg·kg～·d沒(méi)藥甾酮灌胃，對(duì)照組
2、大鼠予生理鹽水灌胃。每5天測(cè)量裸鼠質(zhì)量和腫瘤體積，處理30d后取材，提取腫瘤組織總RNA并分離miR—NA。采用微陣列雜交，通過(guò)芯片掃描和數(shù)據(jù)分析獲得miRNAs表達(dá)譜。結(jié)果高、低劑量實(shí)驗(yàn)組大鼠移植瘤體積分別在處理20d和25d后顯著低于對(duì)照組(P<0．01)；處理30d后高、低劑量組移植瘤質(zhì)量均顯著低于對(duì)照組(P<0．01)。芯片結(jié)果顯示，高劑量實(shí)驗(yàn)組瘤組織miRNAs表達(dá)譜與對(duì)照組相比有顯著差異。結(jié)論沒(méi)藥甾酮可以顯著抑制膽管癌RBE細(xì)胞株移植瘤生長(zhǎng)并影響其miRNA的表達(dá)。關(guān)鍵詞：沒(méi)藥甾酮；膽
3、管癌；移植瘤；microRNAsdoi：10．3969／j．issn．1009—6469．2015．04．007EfectsofguggulsteroneongrowthandmicroRNAsexpressionprofileintransplantedtumorofcholangiocarcinomaRBEcellsZHONGFei，TONGZhu—ring，F(xiàn)ANLu—lu，etal(DepartmentofOncology，F(xiàn)intAffiliatedHospital，AnhuiMedic
4、alUniversity，Hefei230022，China)AbstractObjectiveTodeterminetheeffectsofguggulsteroneonthegro~handmicroRNAsexpressionprofileintransplantedtumorofhumancholangiocarcinomaRBEcells．MethodsFemaleSDnudemicewereinjectedwithRBEcellstoestablishtransplantedtumor
5、models．Themicewereequallyrandomizedinto3groupsincludingcontrolgroup，highdoseandlowdosetreatmentgroups．Oraladmin—istrationofguggulsterane[0，40and20mg·kg一·drespectively]weregiventothecorrespondingmicein72haftertransplanta—tion．Tumorvolumesandweightswere
6、measuredevery5d．Miceweresacrificedafter30days’treatment．TotalRNAwasextractedandmiRNAswereisolatedandhybridizedwithmiRNAsmicroarrays．ResultsThegro~hoftransplantedtumo~wassignificantlysup—pressedintreatmentgroups．Tumorsvolumeweresignificantlysmallerinhi
7、ghdosegroupandlowdosegroupthaninthecontrolafter20days’(P<0．01)and25days’treatment(P<0．01)．ThemicroarrayassayshowedsignificantlydifferentmiRNAsexpressionintumorsofhi【ghdosegroupcomparedwiththecontrolgroupConclusionGuggulsteronesignificantlysuppressesth
8、egrowthoftransplan·tedtumorofhumanch0lanocarcinomaRBEcellsandaffectmicroRNAsexpressionprofile．Keywords：guggulsterone；cholangiocarcinoma；transplantedtumor；microRNAs小分子RNA(microRNA，miRNA)是一類(lèi)廣泛左右的非編碼單鏈小分子RNA，它們通過(guò)介導(dǎo)轉(zhuǎn)錄存在于動(dòng)物和植物等多細(xì)胞生物中約22個(gè)核苷酸后基因沉默，在生
