慢性間歇低氧對大鼠血脂和ghrelin表達(dá)的影響.pdf

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1、p華老年心腦血管病雜志2o14年7月第l6卷第7期ChinJGeriatrHeartBrainVesselDis，Jul2o14，Vol16，No．7·747·基礎(chǔ)研究幔性間歇低氧對大鼠血脂和ghrelin表達(dá)的影響陳雷，孫曉，王實，佟浩，張曼摘要：目的觀察慢性間歇低氧對大鼠血脂及血管內(nèi)皮和下丘腦ghrelin表達(dá)的影響。方法將健康雄性Wistar大鼠60只，隨機(jī)分為正常空氣組，持續(xù)低氧組和間歇低氧組，每組2o只，第l、3個月末測大鼠血脂，C反應(yīng)蛋白(CRP)，RT—PCR法測下丘腦和血管內(nèi)皮ghrelin基因表達(dá)，

2、Westernblot法測血管內(nèi)皮血管細(xì)胞黏附分子1(VCAM一1)、細(xì)胞間黏附分子1(ICAM一1)蛋白表達(dá)。結(jié)果與正?？諝饨M比較，持續(xù)低氧組血脂、CRP無顯著變化(P>0．05)，間歇低氧組血脂、CRP明顯增加(P0．05)，間歇低氧組下丘腦和血管內(nèi)皮ghrelin基因表達(dá)明顯降低(0．524-0．28vs0．68士0．41，0．694

3、-0．21哪!．19±0．21，P<0．05)；與正?？諝饨M比較，持續(xù)低氧組vCAMl、IcAM一1蛋白表達(dá)無明顯變化(P>0．05)，間歇低氧組VCAM一1、ICAM一1蛋白表達(dá)明顯增加(P

4、Lei，SUNXiao，WANGShi，eta1(DepartmentofCardiology，Af}iliatedCentralHospitalofShenyangMedicalCollege，Shenyang110024，LiaoningProvine，China)Abstract：ObjectiveTostudytheeffectofchronicintermittenthypoxemiaonlipidlevelandghre—linexpressioninendotheliumandhypothalamuso

5、frats．MethodsSixtyhealthymaleWistarratswererandomlydividedintonormalgroup，persistenthypoxemiagroup，andintermittenthypox—emiagroup．Theirbodyweight，serumlipidandCRPlevelweremeasured，expressionsofghrelin，VCAM一1andICAM一1proteininhypothalamusandendotheliumweredetect

6、edbyRT—PCRandWesternblot，respectively，attheendofmonths1and3．ResultsNosignificantdifferencewasfoundinbodyweight，serumlipidandCRPlevelbetweennormalgroupandpersistenthypoxemiagroup(P>0．05)．Thebodyweight，serumlipidandCRPlevelweresignificantlyhigherininter—mittenthy

7、poxemiagroupthaninnormalgroup(P<0．05)．Nosignificantdifferencewasfoundinghrelinexpressionlevelbetweennormalgroupandpersistenthypoxemiagroup(P>0．05)．Theghrelinexpressionlevelwassignificantlylowerinintermittenthypoxemiagroupthaninnormalgroup(P<0．05)．Nosignificantd

8、ifferencewasfoundinVCAM一1andICAM一1expressionlevelbetweenpersistenthypoxemiagroupandnormalgroup(P~0．05)．TheVCAM一1andICAM一1ex—pressionlevelsweresignificantlyhigherinintermitte