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《奈達(dá)鉑體外誘導(dǎo)人卵巢癌順鉑耐藥細(xì)胞凋亡的機(jī)制-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、·1216·腫瘤防治研究2012年第39卷第10期doi：10．3971／J．issn．i000—8578．2012．10．013奈達(dá)鉑體外誘導(dǎo)人卵巢癌順鉑耐藥細(xì)胞凋亡的機(jī)制王莉，劉漢鋒，蔡正文，藍(lán)東，覃海園NedaplatinInducedApoptosisinHumanOvarianCancerCisplatin—resistantCellLinesinvitroandInvestigatedItsMechanismWangLi，LiuHanfeng，CaiZhengwen，1．a(chǎn)nDong，QinHaiyuanDepartmentofMedicalOocology，TheFi
2、rstAffiliatedHospitalofGuangxiMedicalUniversity，Nanning，530007，ChinaCorrespondingAuthor：LiuHanfeng，E-mail：237／)78725q．cornAbstract：ObjeetiveTostudyinhibitionofnedaplatin(NDP)onhumanovariancancercelllinesSKOV3withintrinsiccisplatin—resistanceandSKOV3／DDPwithacquiredcisplatin—resistanceandinvest
3、igateitsmechanism．MethodsMTTassaywasusedtoevaluatetheinhibitionindifferentconcentrationandtime0fNDPtreatmentonSKOV3andSKOV3／DDPcells，thencalculatedinhibitoryconcentration(IC．．．)．Cellapoptosisandcycleweredetectedbyflowcytometry(FCM)．ThechangesofBcl2，Bax，caspase-3，caspase-9．MMP-2inmRNAlevelswere
4、quantifiedbyRT—PCR．ResultsNDPinhibitedthegrowthofSKOV3andSKOV3／DDPcellssignificantlyintimeanddose-dependentmanner．TheresultfromFCMshowedthatapoptoticrateandthepercentageofS-phasecellstendtoincreaseintimeandconcentrationofnedaplatindependentmanner(P<0．05)．Comparedwithcontrolgroups，therewereadec
5、reaseinBcl2andMMP-2mRNAexpression，butanincreaseinBax，caspase一3andcaspase-9．ConclusionNDPcaninhibitproliferationofSKOV3andSKOV3／DDPcells．Theeffectmaybeascribedtoinducingcellapopto—sis，theS-phasearrestandthedownregulationofBcl2，MMP一2expression，up—regulationofBax，caspase一3andcaspase-9expression．N
6、DPcansuppressMMP2expression，soitmaybecanreduceinvasionforceofovariancancer．Keywords：Nedaplatin；SKOV3；SKOV3／DDP；Cellapoptosis；Cisplatinresistance摘要：目的探討研究奈達(dá)鉑(NDP)體外對(duì)人卵巢癌順鉑原發(fā)耐藥細(xì)胞株sK()V3和繼發(fā)耐藥株SKOV3／DDP的抑制作用及其機(jī)制。方法采用MTT法檢測(cè)體外不同時(shí)間不同濃度的NDP對(duì)SKOV3及SKOV3／DDP細(xì)胞的殺傷作用，計(jì)算半數(shù)抑制濃度(ICs，)；流式細(xì)胞術(shù)(FCM)測(cè)定給藥前后細(xì)胞凋亡率及周
7、期分布變化；半定量PCR分析凋亡基因Bcl一2，Bax，caspase3，caspase-9及反應(yīng)腫瘤細(xì)胞侵襲力的基因MMP一2的表達(dá)變化。結(jié)果NDP能明顯抑制sKOV3及sKOv3／DDP生長(zhǎng)，呈時(shí)間一劑量依賴性；流式結(jié)果顯示隨時(shí)間、濃度增加，SKOV3和SK0V3／DDP細(xì)胞凋亡明顯增加，S期細(xì)胞的比例增加，差異均有統(tǒng)計(jì)學(xué)意義(P
