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1、葉下珠提取物抑菌活性探究 摘要：采用生長速率法測定葉下珠（PhyllanthusurinariaLinn）的5種溶劑提取物對大腸桿菌[Escherichiacoli（Migula）Casleillii&Chalmers]、傷寒沙門氏菌[Salmonellatyphi（Schroeter）Warren&Scott]、痢疾志賀氏菌[Shigelladysenteriae（Shiga）Castellani&Chalmers]、蠟狀芽孢桿菌（BacilluscereusFrankland）、金黃色葡萄球菌（StaphyloccocusaureusRos
2、enbach）的抑制活性。結(jié)果表明，10mg/mL葉下珠甲醇提取物、乙醇提取物和丙酮提取物對痢疾志賀氏菌、金黃色葡萄球菌和傷寒沙門氏菌有較強的抑制活性，其抑菌率均不低于60%，對大腸桿菌和蠟狀芽孢桿菌的抑制作用較弱，抑菌率均不高于60%；10mg/mL水提取物和石油醚提取物對5種細(xì)菌的抑制作用弱，抑菌率均在51%以下。比較各提取物對5種供試菌的毒力可見，甲醇提取物對痢疾志賀氏菌的抑菌活性最強，其抑制有效中濃度（EC50）為2.9155mg/mL，乙醇提取物、丙酮提取物對金黃色葡萄球菌的抑菌活性最強，EC50分別為3.4402、4.3206mg/m
3、L。關(guān)鍵詞：葉下珠；提取物；抑菌活性中圖分類號：R285.5文獻(xiàn)標(biāo)識碼：A文章編號：0439-8114（2013）12-2812-037StudyonAntibacterialActivitiesofExtractsfromPhyllanthusurinariaLinnDENGZhi-yong（CollegeofChemicalandBiologicalEngineering，HezhouUniversity，Hezhou542800，Guangxi，China）Abstract：Theantibacterialactivitiesof5solv
4、entextractsfromPhyllanthusurinariaLinnagainst5speciesofbacteriumweredeterminedbygrowthratemethod.Theresultsshowedthat3solventwhichweremethanol，ethanolandacetoneextractshadhighantibacterialactivitiesShigelladysenteriae（Shiga）Castellani&Chalmers，StaphyloccocusaureusRosenbach，Sa
5、lmonellatyphi（Schroeter）Warren&Scottwithantibacterialratesmorethan60%，andhadlowantibacterialactivitiesagainstEscherichiacoli（Migula）CasleilliiandChalmers，BacilluscereusFranklandwithantibacterialratesbelow60%.Thewaterextractsandpetroleumetherextractshadlowantibacterialactiviti
6、esagainst5speciesofbacteriumwiththeantibacterialratesbelow51%.Themethanolextractshadthehighestantibacterial7activitiesagainstShigelladysenteriae（Shiga）Castellani&Chalmers，whoseEC50was2.9155mg/mL.TheethanolextractshadthehighestantibacterialactivitiesagainstStaphyloccocusaureus
7、Rosenbach，whichEC50was3.4402mg/mL.TheacetoneextractshadthehighestantibacterialactivitiesagainstStaphyloccocusaureusRosenbach，whoseEC50was4.3206mg/mL.Keywords：PhyllanthusurinariaLinn；extracts；antibacterialactivities植物源殺菌劑是指從植物中提取的具有殺菌、抑菌活性的物質(zhì)，以及由其分離純化的單體物質(zhì)加工而成的藥劑。從植物中尋找殺菌、抑菌活性
8、物質(zhì)已成為當(dāng)今開發(fā)、研制無公害新型殺菌劑的熱點之一[1]。葉下珠（PhyllanthusurinariaLinn）為大戟科（Euphor
