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1、中國水稻科學(ChinJRiceSci),2015,29(1):14-21http://www.ricesci.cn14DOI:10.3969/ji.ssn.1001G7216.2015.01.002水稻白條紋葉突變體st11的遺傳分析與基因定位成欽淑#葉邦全#袁燦#李偉滔尹俊杰王靜賀閩汪吉春王玉平李仕貴陳學偉?(四川農(nóng)業(yè)大學水稻研究所,成都611130;#共同第一作者;?通訊聯(lián)系人,EGmail:xwchen88@163.com)GeneticAnalysisandGeneMappingofWhiteStripeLeafMutantst11inRiceCHENGQin
2、Gshu#,YEBangGquan#,YUANCan#,LIWeiGtao,YINJunGjie,WANGJing,HEMin,WANGJiGchun,WANGYuGping,LIShiGgui,CHENXueGwei?(RiceResearchInstitute,SichuanAgriculturalUniversity,Chendu611130,China;#Theseauthorscontributedequallytothiswork;?Correspondingauthor,EGmail:xwchen88@163c.om)CHENGQinshu,YEBangq
3、uan,YUANCan,etal.Geneticanalysisandgenemappingofwhitestripeleafmutantst11inrice.ChinJRiceSci,2015,29(1):14G21.Abstract:Awhitestripeleafmutantst11wasobtainedfromtissueGculturedriceKitaake(Oryzasativasppjaponica).Thismutantwascharacterizedbywhitestripeleavesfromtilleringstagetomaturestage.
4、ComparedwiththewildtypeKitaake,themutantshowednoobviouschangesinagriculturaltraits,suchastillernumber,plantheight,seedGsettingrate,andgrainweight.Geneticanalysesshowedthatthewhitestripeleafphenotypeinthemutantst11wascontrolledbyasinglerecessivegene.TwoF2segregatingpopulationsderivedfromt
5、hecrosses,st11×Jodanandst11×02428,respectively,wereusedformappingthegenest11.Bulkedsegregantanalysissuggestedthatthegenest11waslocatedclosetothemarkers,RM151andRM10080,onchromosome1.Usingmoremolecularmarkers,wefinallydelimitedthegenest11toaregionofabout270kbbetweentheIndelmarkers,I10andI
6、26,onthetelomereoftheshortarmofchromosome1.Keywords:rice;whitestripeleafmutant;molecularmarker;genemapping成欽淑,葉邦全,袁燦,等.水稻白條紋葉突變體st11的遺傳分析與基因定位.中國水稻科學,2015,29(1):14G21.摘要:白條紋葉突變體st11是從粳稻品種Kitaake組培過程中獲得的.該突變體在分蘗前葉色表現(xiàn)為正常,從分蘗期開始新生葉表現(xiàn)為白條紋直至成熟期.與野生型相比,該突變體的分蘗、株高、結(jié)實率和千粒重等農(nóng)藝性狀沒有發(fā)生明顯變化.遺傳分析表明該突變
7、體白條紋葉性狀受一對隱性核基因控制.利用該突變體分別與水稻02428、Jodan雜交構(gòu)建了兩個F2群體用于基因定位.通過集群分離分析(bulkedsegregantanalysis)發(fā)現(xiàn)該基因位于第1染色體端粒附近,并與分子標記RM151和RM10080連鎖.進一步利用更多分子標記分析F2群體,我們將該基因定位于I10和I26兩個標記之間大約270kb的區(qū)間內(nèi).關(guān)鍵詞:水稻;白條紋葉突變體;分子標記;基因定位中圖分類號:Q343?5;S754文獻標識碼:A文章編號:1001G7216(2015)01G0014G08水稻是我國乃至世界的重要