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1、中國(guó)水稻科學(xué)(ChinJRiceSci),2017,31(4):355－363http://www.ricesci.cnDOI:10.16819/j.1001-7216.2017.6165355水稻白條紋突變體st13的表型分析及基因定位1,31,3111111,2,*孫立亭林添資王云龍牛梅胡婷婷劉世家王益華萬(wàn)建民1(南京農(nóng)業(yè)大學(xué)作物遺傳與種質(zhì)創(chuàng)新國(guó)家重點(diǎn)實(shí)驗(yàn)室/農(nóng)業(yè)部長(zhǎng)江中下游粳稻生物學(xué)與遺傳育種重點(diǎn)實(shí)驗(yàn)室/長(zhǎng)江流域雜交水稻協(xié)同創(chuàng)新中心/江蘇省23現(xiàn)代作物生產(chǎn)中心，南京210095；中國(guó)農(nóng)業(yè)科學(xué)院
2、作物科學(xué)研究所，北京100081；江蘇丘陵地區(qū)鎮(zhèn)江農(nóng)業(yè)科學(xué)研究所，江蘇句容212400；*通訊聯(lián)系人，E-mail:wanjm@njau.edu.cn,wanjianmin@caas.cn)PhenotypicAnalysisandGeneMappingofaWhiteStripeMutantst13inRice1,31,311111SUNLiting,LINTianzi,WANGYunlong,NIUMei,HUTingting,LIUShijia,WANGYihua,1,2,*WANJianmi
3、n1(StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniversity/KeyLaboratoryofBiology,GeneticsandBreedingofjaponicaRiceinMid-lowerYangtzeRiver,MinistryofAgriculture/TheYangtzeRiverValleyHybridRiceCollaborationInnovationCenter/
4、Jiangsu2CollaborationInnovationCenterforModernCropProduction,Nanjing210095,China;InstituteofCropScience,ChineseAcademyofAgriculturalSciences,3*Beijing100081,China;ZhenjiangAgriculturalResearchInstitute,Jurong212400,China;Correspondingauthor,E-mail:wanj
5、m@njau.edu.cn,wanjianmin@caas.cn)Abstract:【Objective】Riceleafcolormutationidentificationandcloningofrelatedgenesishelpfultoresearchphotosynthesisandfurtherunderstandthemechanismofchloroplastdevelopmentandpigmentsynthesispathway,providingtheoreticalbasi
6、sforthebreedingofricewithhighphotosyntheticefficiency.【Method】Asomaclonalmutant(designatedasstripeleaf13,st13)wasisolatedfromtissue-culturedprogeniesofajaponicaricevarietyDongjin.Themainagronomictraitsofwildtypeandst13weredeterminedatmaturity,andthepig
7、mentcontentsandultrastructureofchloroplastwereobservedattheseedlingstage.Dongjinandst13werereciprocallycrossedtoobservethephenotypeofF1andgeneticanalysiswascarriedout.AnF2populationandF2:3populationderivedfromthecrossst13×Nanjing11(anindicaricevariety)
8、wereusedforgenemappingofST13.QuantitativeRT-PCRwascarriedouttoanalyzetherelativeexpressionofgenesassociatedwithchloroplastdevelopmentandchlorophyllbiogenesisinthewild-typeDongjinandst13mutant.【Result】Comparedtothewildtype,theplantheight
