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1、中國水稻科學(ChinJRiceSci),２０１６,３０(５):４４７－４５７http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．６０４８４４７水稻粉質(zhì)胚乳突變體ws的表型分析及基因克隆潘鵬屹朱建平王云龍郝媛媛蔡躍張文偉江玲王益華萬建民?(南京農(nóng)業(yè)大學作物遺傳與種質(zhì)創(chuàng)新國家重點實驗室/農(nóng)業(yè)部長江中下游粳稻生物學與遺傳育種重點實驗室/長江流域雜交水稻協(xié)同創(chuàng)新中心/江蘇省現(xiàn)代作物生產(chǎn)中心,南京２１００９５;?通訊聯(lián)系人,EＧmail:wanjm＠nja
2、u．edu．cn)PhenotypingandGeneCloningofaFlouryEndospermMutantwsinRicePANPengＧyi,ZHUJianＧping,WANGYunＧlong,HAOYuanＧyuan,CAIYue,ZHANGWenＧwei,JIANGLing,WANGYiＧhua,WANJianＧmin?(StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniver
3、sity/KeyLaboratoryofBiology,GeneticsandBreedingofjaponicaRiceinMidＧlowerYangtzeRiver,MinistryofAgriculture/TheYangtzeRiverValleyHybridRiceCollaborationInnovationCenter/JiangsuCollaborativeInnovationCenterforModernCropProduction,Nanjing２１００９５,China;?Co
4、rrespondingauthor,EＧmail:wanjm＠njaue．duc．n)PANPengyi,ZHUJianping,WANGYunlong,etal．Phenotypingandgenecloningofaflouryendospermmutantwsinrice．ChinJRiceSci,２０１６,３０(５):４４７Ｇ４５７．Abstract:Inthisstudy,weobtainedastablyinheritedflouryendospermmutantwsfromjapon
5、icavarietycv．Dianjingyou１(DJY),withits１０００Ｇgrainweight,grainsize,totalstarchcontent,andamylosecontentinmatureseedsalldecreasedcomparedwithwildＧtype,aswellastheswellingpowerofstarch．Byobservingthestructureofmatureanddevelopingendosperm,wefoundthatthest
6、archgranulesinwsmutantweresmallerandlooselypacked．TheWSlocuswasfirstmappedtoaregionnearthecentromereinchromosome８with９２recessiveindividuals,andthenwasnarroweddowntoa９５kbregionbyusing２０２５recessiveindividuals．Sequenceanalysisrevealedthatthecodingregiono
7、fthesmallsubunitofadenosinediphosphateglucosepyrophosphorylase(AGPS２)hadasinglenucleotidesubstitution,resultinginachangeintheaminoacidsequence．RTＧPCRanalysisshowednosignificantdifferenceintheexpressionlevelsofgenesencodingtheAGPasesubunitsinthemutante
8、ndosperm,whileimmunoblotanalysisrevealedareducedproteincontentoftheAGPS２b．Meanwhile,theenzymeactivityofAGPaseinthemutantwasdecreasedtohalfofthewildＧtype．TheseresultsshowedthatthemutationofOsAGPS２causedthedecreasedactivityofAGPase,thusaffecting
