[農(nóng)林牧漁]plant genomics methods and protocols

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1、PlantGenomicsMethodsandProtocolsEditedbyDarylJ.Somers*,PeterLangridge?,andJ.PerryGustafson?MolecularBreedingandBiotechnology,VinelandResearchandInnovationCentre,VinelandStation,Ontario,Canada*AustralianCentreforPlantFunctionalGenomics,UniversityofAdelaide,GlenOsmond,

2、Australia?DivisionofPlantSciences,UniversityofMissouri,Columbia,MO,USA?PrefaceThisvolumeisdividedintochapterswhichconsidertheprimaryissuesandmethodologiessurroundingplantgenomicsresearch.Plantgenomicsislargelyconcernedwithassociatingfunctionalgenesorgenemutationswith

3、phenotype.Therefore,chaptersareincludedthatcovertheareasofgenediscoveryandfunctionalanalysisofgenes.Furtherchaptersfocusontheprimarytoolsandsub-disciplinesofgeneticmapping,mRNA,proteinandmetaboliteprofiling.Methodsareincludedthatexploregenefunctionalanalysisviatransf

4、ormation,mutation,proteinfunctionandgeneexpression.Thevolumeincludeschaptersondatamanagementwhichconsidertheexpansionofplantgenomicsdatabasesandbioinformaticsanalysistools.Thevolumeisconcludedwithchaptersaimedatdiscussingtheapplicationanddeploymentofmolecularplantbre

5、edingtechnologyfromtheuseofmarkersinbreeding,developmentofgeneticallymodifiedplants/cropspecies,analysisofexistingpopulationsfornovelallelesandgene/traitassociationsandgenomesequencing.ContentsPreface............................................................vContri

6、butors........................................................ix1RoleofModelPlantSpecies.........................................1RichardFlavell2NewTechnologiesforUltra-HighThroughputGenotypinginPlants..........19NikkiAppleby,DavidEdwards,andJacquelineBatley3GeneticM

7、apsandtheUseofSynteny..................................41ChrisDuran,DavidEdwards,andJacquelineBatley4ASimpleTAE-BasedMethodtoGenerateLargeInsertBACLibrariesfromPlantSpecies.....................................57Bu-JunShi,J.PerryGustafson,andPeterLangridge5TranscriptP

8、rofilingandExpressionLevelMapping........................81ElenaPotokina,ArnisDruka,andMichaelJ.Kearsey6MethodsforFunctionalProteom