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1、中華人民共和國出入境檢驗檢疫行業(yè)標準SNIT1155-2002植物檢疫玉米霜霉病菌檢疫鑒定方法Plantquarantine-MethodsforquarantineandidentificationofPeronosclerospora(lto)C.G.Shaw2002一11一25發(fā)布2003一05一01實施中華人民共和國發(fā)布國家質(zhì)量監(jiān)督檢驗檢疫總局SN/T1155-2002目次前言···?！ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁ?，······‘·····，···。·····················，·········，，···
2、···············，···············，····一I1范圍····································，···········，······························································??12原理···································································，·······························，··，·····一13儀器
3、和用具······································································，···························??14試劑和材料····································································································??15現(xiàn)場檢疫與取樣‘。?！ぁぁぁぁ?，·····‘·················，··········，·，····
4、···，，，，，，··，，，，·，····，，，，···??26實驗室檢驗··············，·······················，··························································，···??37結(jié)果評定·····························，······························，··········，··??‘············，·??58樣品保存·········。················
5、······························，················································??5附錄A(資料性附錄)玉米霜霉病菌寄主范圍·························································??6附錄B<資料性附錄)DNA提取、擴增與電泳，···························，························，··??7附錄C(規(guī)范性附錄)PCR擴增引物·...........
6、.....................................................“一’二’“9附錄D(資料性附錄)玉米霜霉病菌卵抱子、分生抱子、分生抱子梗圖···························??10SN/T1155-2002.幾J..叫.-月Ili昌本標準的附錄C為規(guī)范性附錄，附錄A、附錄B、附錄D為資料性附錄。本標準由國家認證認可監(jiān)督管理委員會提出并歸口。本標準起草單位:中華人民共和國福建出人境檢驗檢疫局。本標準主要起草人:李德福、郭瓊霞、陳艷、張廣志、張曉燕。本標準系首次發(fā)布的檢驗檢疫行
7、業(yè)標準。SN/T1155-2002植物檢疫玉米霜霉病菌檢疫鑒定方法范圍本標準規(guī)定了植物檢疫中玉米霜霉病菌檢疫和鑒定方法。本標準適用于玉米、甘蔗、高粱及其他寄主中玉米霜霉病菌的檢疫和鑒定。2原理玉米霜霉病主要由高粱霜霉病菌(Peronosclerosporasorghi)、甘蔗霜霉病菌(P.sacchari)、菲律賓霜霉病菌(P.philippinen.sis)和爪哇霜霉病菌(P.maydis)引起，該類病菌(以下簡稱玉米霜霉病菌Peronosclerosporaspp.)屬鞭毛菌亞門(Mastigomycotina),夢p菌綱(Oomy
8、cetes),霜霉目(Peronospora-les),霜霉科(Peronosporaceae)、霜指霉屬(Peronosclerospora)。玉米霜霉病菌除危害玉米外，還侵染其他植物(參見附錄A)。一般
