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《納米雄黃對(duì)皮膚鱗癌a431細(xì)胞株抑制增殖及抑制新生血管作用的實(shí)驗(yàn)研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、提要目的：對(duì)皮膚腫瘤進(jìn)行中醫(yī)文獻(xiàn)復(fù)習(xí)，并通過檢測人皮膚鱗癌A431細(xì)胞用納米雄黃處理后細(xì)胞增殖抑制及血管生長調(diào)控因子表達(dá)情況，探討納米雄黃抑制血管生長抗腫瘤的作用機(jī)理。方法：查詢并復(fù)習(xí)中醫(yī)文獻(xiàn)資料，對(duì)皮膚鱗癌A431細(xì)胞進(jìn)行體外培養(yǎng)，按實(shí)驗(yàn)分組加藥后觀察其形態(tài)及生長狀況變化，并分別用MTT法、免疫組織化學(xué)法和RT-PCR法檢測加藥后細(xì)胞增殖抑制、色素上皮源性因子(PEDF)和VEGFmRNA表達(dá)情況，探討納米雄黃抑制皮膚鱗癌新生血管生成的作用機(jī)理。結(jié)果：納米雄黃可以抑制人皮膚鱗癌A431細(xì)胞增殖，且與順鉑聯(lián)合應(yīng)用有協(xié)同作用，并發(fā)現(xiàn)納米雄黃抑制A431細(xì)胞增殖的作用與上調(diào)PEDF的表
2、達(dá)和下調(diào)VEGF的表達(dá)以抑制新生血管生成有關(guān)。結(jié)論：體外實(shí)驗(yàn)表明納米雄黃可顯著抑制人皮膚鱗癌A431細(xì)胞增殖，并通過抑制其新生血管生成達(dá)到抗腫瘤細(xì)胞生長的效果，與順鉑有協(xié)同作用。關(guān)鍵詞納米雄黃；人皮膚鱗狀細(xì)胞癌A431細(xì)胞；腫瘤血管生成；細(xì)胞增殖ExperimentalResearchofRealgarnanoparticlesoncutaneoussquamousCellcarcinomaintheInhibitionofproliferationandNeovascularizationsuppressionSpeciality:ClinicalcombinationMedic
3、ineofTCMWithWesternMedicineAuthor:LinYanyanTutor:ProfessorLiXiurongAbstractObjective:ReviewtheliteratureofTCMonskintumor.DetectthecellproliferationandbloodvessalgrowthregulatoryfactorexpressionofskinsquamouscellcarcinomaA431cellstreatedwithnanometer,thenexploretherealgarnanoparticlestoanti-tum
4、ormechanismofaction.Methods:SkinsquamouscellcarcinomaA431cellswereculturedinvitro.Doseintheexperimentalgroupsandobservethechangesinitsmorphologyandgrowthconditions,respectively,usingtheMTTassay,immunohistochemistryandRT-PCRassaydosinginhibitionofcellproliferation,theexpressionofpigmentepitheli
5、um-derivedfactor(PEDF)andVEGFmRNA.Results:RealgarnanoparticlescaninhibithumanskinsquamouscellscarcinomaA431cellsproliferationandsynergywithcisplatin.Realgarnanoparticlescanru-regulatetheexpressionofPEDFanddowntheexpressionofVEGFtoinhibitangiogenesis.Conclusion:InvitroexperimentsshowedRealgarna
6、noparticlescansignificantlyinhibithumanskinsquamouscellcarcinomaA431cellsproliferationandtreatcancerbyinhibitingangiogenesisgeneratesynergywithcisplatin.KeywordsRealgarnanoparticles;SkinsquamouscellcarcinomaA431cells;inhibitingangiogenesis;Inhibitionofproliferation目錄引言.........................
7、......................................................................................................1第一部分實(shí)驗(yàn)研究.............................................................................................................3一實(shí)驗(yàn)方法和結(jié)果.......
