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《納米雄黃對皮膚鱗癌a431細胞株抑制增殖及抑制新生血管作用的實驗研究》由會員上傳分享，免費在線閱讀，更多相關內容在學術論文-天天文庫。
1、提要目的：對皮膚腫瘤進行中醫(yī)文獻復習，并通過檢測人皮膚鱗癌A431細胞用納米雄黃處理后細胞增殖抑制及血管生長調控因子表達情況，探討納米雄黃抑制血管生長抗腫瘤的作用機理。方法：查詢并復習中醫(yī)文獻資料，對皮膚鱗癌A431細胞進行體外培養(yǎng)，按實驗分組加藥后觀察其形態(tài)及生長狀況變化，并分別用MTT法、免疫組織化學法和RT-PCR法檢測加藥后細胞增殖抑制、色素上皮源性因子(PEDF)和VEGFmRNA表達情況，探討納米雄黃抑制皮膚鱗癌新生血管生成的作用機理。結果：納米雄黃可以抑制人皮膚鱗癌A431細胞增殖，且與順鉑聯合應用有協同作用，并發(fā)現納米雄黃抑制A431細胞增殖的作用與上調PEDF的表
2、達和下調VEGF的表達以抑制新生血管生成有關。結論：體外實驗表明納米雄黃可顯著抑制人皮膚鱗癌A431細胞增殖，并通過抑制其新生血管生成達到抗腫瘤細胞生長的效果，與順鉑有協同作用。關鍵詞納米雄黃；人皮膚鱗狀細胞癌A431細胞；腫瘤血管生成；細胞增殖ExperimentalResearchofRealgarnanoparticlesoncutaneoussquamousCellcarcinomaintheInhibitionofproliferationandNeovascularizationsuppressionSpeciality:ClinicalcombinationMedic
3、ineofTCMWithWesternMedicineAuthor:LinYanyanTutor:ProfessorLiXiurongAbstractObjective:ReviewtheliteratureofTCMonskintumor.DetectthecellproliferationandbloodvessalgrowthregulatoryfactorexpressionofskinsquamouscellcarcinomaA431cellstreatedwithnanometer,thenexploretherealgarnanoparticlestoanti-tum
4、ormechanismofaction.Methods:SkinsquamouscellcarcinomaA431cellswereculturedinvitro.Doseintheexperimentalgroupsandobservethechangesinitsmorphologyandgrowthconditions,respectively,usingtheMTTassay,immunohistochemistryandRT-PCRassaydosinginhibitionofcellproliferation,theexpressionofpigmentepitheli
5、um-derivedfactor(PEDF)andVEGFmRNA.Results:RealgarnanoparticlescaninhibithumanskinsquamouscellscarcinomaA431cellsproliferationandsynergywithcisplatin.Realgarnanoparticlescanru-regulatetheexpressionofPEDFanddowntheexpressionofVEGFtoinhibitangiogenesis.Conclusion:InvitroexperimentsshowedRealgarna
6、noparticlescansignificantlyinhibithumanskinsquamouscellcarcinomaA431cellsproliferationandtreatcancerbyinhibitingangiogenesisgeneratesynergywithcisplatin.KeywordsRealgarnanoparticles;SkinsquamouscellcarcinomaA431cells;inhibitingangiogenesis;Inhibitionofproliferation目錄引言.........................
7、......................................................................................................1第一部分實驗研究.............................................................................................................3一實驗方法和結果.......
