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1、PlenarypaperDynamictrackingofhumanhematopoieticstemcellengraftmentusinginvivobioluminescenceimagingXiuliWang,MichaelRosol,ShundiGe,DenisePeterson,GeorgeMcNamara,HarveyPollack,DonaldB.Kohn,MarvinD.Nelson,andGayM.CrooksThestandardapproachtoassesshemato-patt
2、ernsofengraftmentkineticsfromhu-miceafterdeathdemonstratedthatlevelspoieticstemcell(HSC)engraftmentinmanCD34andCD34CD38populationsof1%donorcellengraftmentcouldbeexperimentalbonemarrowtransplanta-inthemarrowNOD/SCID/2mnullmice.Imag-readilydetectedbybio
3、luminescenceim-tionmodelsreliesondetectionofdonoringshowedanearlypeak(day13)ofen-aging;higherengraftmentlevelscorre-hematopoieticcellsinhostbonemarrowgraftmentfromCD34cellsfollowedbyaspondedtohigherimagesignalintensity.followingdeath;thisapproachprovides
4、rapiddeclineinsignal.EngraftmentfromInvivobioluminescenceimagingpro-datafromonlyasingletimepointafterthemoreprimitiveCD34CD38popula-videsanovelmethodtotrackthedynam-transplantationforeachanimal.Invivotionwasrelativelydelayedbutbyday36icsofengraftmentofh
5、umanHSCandbioluminescenceimagingwasthereforeincreasedtosigni?cantlyhigherlevelsprogenitorsinvivo.(Blood.2003;102:exploredasamethodtogainadynamic,thanthosefromCD34cells(P<.05).3478-3482)longitudinalpro?leofhumanHSCengraft-SignalintensityfromCD34CD38-men
6、tinalivingxenogeneicmodel.Lucif-engraftedmicecontinuedtoincreasedur-eraseexpressionusingalentiviralvectoringmorethan100daysofanalysis.Flowalloweddetectionofdistinctlydifferentcytometryanalysisofbonemarrowfrom?2003byTheAmericanSocietyofHematologyIntroducti
7、onKeyaspectsofthebiologyofhematopoieticstemcells(HSCs)Wenowshowthatbyaccomplishinghighef?ciency,stablehavebeenstudiedformanyyearsusingelegantmurinemodelsoftransduction,andexpressionofluciferaseinHSCs,biolumines-bonemarrowtransplantation.Morerecently,immun
8、ode?cientcenceimagingcanrevealnovel,dynamicinformationonthemurinemodelshavebeenusedsuccessfullytostudyhumanHSCs.1patternofhumanHSCandprogenitorengraftmentandprolifera-Inallofthesemodels,HSCsareinfusedintorecipientan
