資源描述：
《hematopoetic stem cells BLOOD》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學術(shù)論文-天天文庫。
1、PlenarypaperDynamictrackingofhumanhematopoieticstemcellengraftmentusinginvivobioluminescenceimagingXiuliWang,MichaelRosol,ShundiGe,DenisePeterson,GeorgeMcNamara,HarveyPollack,DonaldB.Kohn,MarvinD.Nelson,andGayM.CrooksThestandardapproachtoassesshemato-patt
2、ernsofengraftmentkineticsfromhu-miceafterdeathdemonstratedthatlevelspoieticstemcell(HSC)engraftmentinmanCD34andCD34CD38populationsof1%donorcellengraftmentcouldbeexperimentalbonemarrowtransplanta-inthemarrowNOD/SCID/2mnullmice.Imag-readilydetectedbybio
3、luminescenceim-tionmodelsreliesondetectionofdonoringshowedanearlypeak(day13)ofen-aging;higherengraftmentlevelscorre-hematopoieticcellsinhostbonemarrowgraftmentfromCD34cellsfollowedbyaspondedtohigherimagesignalintensity.followingdeath;thisapproachprovides
4、rapiddeclineinsignal.EngraftmentfromInvivobioluminescenceimagingpro-datafromonlyasingletimepointafterthemoreprimitiveCD34CD38popula-videsanovelmethodtotrackthedynam-transplantationforeachanimal.Invivotionwasrelativelydelayedbutbyday36icsofengraftmentofh
5、umanHSCandbioluminescenceimagingwasthereforeincreasedtosigni?cantlyhigherlevelsprogenitorsinvivo.(Blood.2003;102:exploredasamethodtogainadynamic,thanthosefromCD34cells(P<.05).3478-3482)longitudinalpro?leofhumanHSCengraft-SignalintensityfromCD34CD38-men
6、tinalivingxenogeneicmodel.Lucif-engraftedmicecontinuedtoincreasedur-eraseexpressionusingalentiviralvectoringmorethan100daysofanalysis.Flowalloweddetectionofdistinctlydifferentcytometryanalysisofbonemarrowfrom?2003byTheAmericanSocietyofHematologyIntroducti
7、onKeyaspectsofthebiologyofhematopoieticstemcells(HSCs)Wenowshowthatbyaccomplishinghighef?ciency,stablehavebeenstudiedformanyyearsusingelegantmurinemodelsoftransduction,andexpressionofluciferaseinHSCs,biolumines-bonemarrowtransplantation.Morerecently,immun
8、ode?cientcenceimagingcanrevealnovel,dynamicinformationonthemurinemodelshavebeenusedsuccessfullytostudyhumanHSCs.1patternofhumanHSCandprogenitorengraftmentandprolifera-Inallofthesemodels,HSCsareinfusedintorecipientan
