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1、RANKL和IL-17A在中耳膽脂瘤中的表達(dá)及相關(guān)性研究田斐李青峰冀永進(jìn)馬敏山西醫(yī)科大學(xué)第二醫(yī)院耳鼻咽喉頭頸外科(太原030001)【摘要】目的觀察破骨細(xì)胞分化因T(RANKL)和白介素-17A(1L-17A)在中耳膽脂瘤組織中的表達(dá)，探討二者在中耳膽脂瘤骨質(zhì)破壞機(jī)制中的作用。方法采用免疫組織化學(xué)兩步法和計(jì)算機(jī)圖像定量分析法，檢測RAN?KL、IL-17A在25例中耳膽脂瘤上皮、16例膽脂瘤周潮肉芽組織及15例正常外耳道皮膚中的表達(dá)，同時(shí)觀察二者的表達(dá)與臨床聽小骨骨質(zhì)破壞程度的相互關(guān)系。結(jié)果(1)RANKL、IL-1
2、7A在膽脂瘤上皮及膽脂瘤周圍肉芽組織中表達(dá)增高，分別與正常對照組比較，差異均有統(tǒng)計(jì)學(xué)意義(?fusxL=9.864.5.630,均戸<0.05；仏加=13.905、9.011,均P<0.05),且二者的表達(dá)與骨質(zhì)破壞程度相關(guān)。(2)在膽脂瘤上皮及膽脂瘤周圍肉芽組織中，RANKL與IL-17A的表達(dá)呈正相關(guān)(riff=0.692,“芽二0.538,Pv0.05)。結(jié)論RANKL及IL-17A在中耳膽脂瘤組織中呈高表達(dá)，與骨質(zhì)破壞程度成正比，表明其與膽脂瘤骨質(zhì)破壞機(jī)制密切相關(guān)；RANKL與IL-17A表達(dá)有相關(guān)性，提示二
3、者之間可能存在相互作用?！娟P(guān)鍵詞］膽脂瘤，中耳；破骨細(xì)胞分化因子；白介素-I7A【中圖分類號】R764.22,R349.39【文獻(xiàn)標(biāo)識碼】A【文章編號】1672-2922(2010)01-090-05ExpressionofRANKLandIL-17AandtheircorrelationinmiddleearcholesteatomaTIANFei,LIQing-feng,JIYong-jin,MAMinDepartmentofOtorhinolaryngology-HeadandNeckSurgetheSecon
4、dHospitalofshanximedicalUniversity^Taiyuan,030001,China[Abstract]ObjectiveTostudyexpressionofRANKL(ReceptorActivatorofNuclearFactor-kappaBLigand)andIL-I7A(Interleukin-17A)inHumanMiddleEarCholestealornaandtheirmlesinbonedestruction.MethodsImmunohistochemicalmeth
5、odsandcomputer-aidedquantitativeimageanalysiswereusedtoexaminetheexpressionofRANKLandIL-17Ain25middleearcholesteatomaspecimens,16granulationtissuesurroundingthecholesteatomaand15specimensofnomiulexternalearcanalftkin.Hiecorrelationbetweenexpressionofthetwoindex
6、esandtheerosionofossicleswerealsoexamined.Results(I)IncreasedrexpressionofthecytokineRANKLandIL-17Awerefoundinthecholesteatomaepitheliumandsurn)un(linggranulationtissue^ascomparedwithnonnalexternalmeatalskin(/rank)=9.864,5.630,P<0.05;fn-i7A=13.905,9.011,P<0.05)
7、.Inaddition,expressionoftheIwoindexeswerecorrelatedtotheextentofossicledestruction.(2)Incholesteatomaepitheliumandgranulationtissue,expressionofRANKLwasdirectlycorrelatedwiththatofIL-17A(P<0.05).ConclusionRANKLandIL-I7Aareoverexpressedinmiddleearcholesteatomaan
8、dtheyareassociatedwithossicledestruction,indicatingthatRANKLandIL-17Aareresponsibleforbonedestructionincholesteatoma;RANKI,andIL-J7Ahaveacorrelationintheirexpression^suggest
