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1、C.R.Biologies334(2011)705–712ContentslistsavailableatScienceDirectComptesRendusBiologieswww.sciencedirect.comGenetics/Ge′ne′tiqueAnalysisofgeneticvariabilityinAristaeomorphafoliacea(Crustacea,Aristeidae)usingDNA-ISSR(InterSimpleSequenceRepeats)markersa,bcbMariaVictoriaFerna′ndez
2、,FerruccioMaltagliati,FedericaG.Pannacciulli,a,*MariaIne′sRolda′naLaboratorid’IctiologiaGene`tica,UniversitatdeGirona,CampusdeMontilivi,17071Girona,SpainbMarineEnvironmentResearchCentre,ENEA–S.Teresa,POBox224,19100LaSpezia,ItalycDipartimentodiBiologia,Universita`diPisa,ViaDerna1
3、,56126Pisa,ItalyARTICLEINFOABSTRACTArticlehistory:Thisworkreportsthe?rstgeneticdataofAristaeomorphafoliacea,amarinedecapodofReceived30April2011highcommercialvalue,fromsixMediterraneanlocalitiesandonenew?shinggroundinAcceptedafterrevision12July2011theAvailableMozambiqueChannel.Th
4、euseof?veInterSimpleSequenceRepeat(ISSR)primersonline7September2011provided150polymorphicloci.Averageestimatesofgeneticdiversitydidnotsigni?cantlydifferamongsampledlocalities,withameanvalueofheterozygosityH=0.1050.015.Keywords:Analysisofmolecularvariance(AMOVA)allocated>98%ofge
5、neticvariabilitytothewithin-MarinedecapodsampleAristaeomorphacomponent,displayingvalueshigherthanthosepreviouslyreportedinISSRstudiesonfoliaceamarineGeneticinvertebrates.Clusteranalysesdidnotdetectgeographicallyorgeneticallydistinctvariabilitygroups.GeneTheobservedlackoflarge-sc
6、alegeneticdifferentiationisdiscussedinrelationtothe?owhighDNA-ISSRspotentialforlarvaldispersalofthespeciesandtofeaturesofthemarkeremployed.?2011Acade′miedessciences.PublishedbyElsevierMassonSAS.Allrightsreserved.1.IntroductionA.foliacea,inordertogiveaninsightintogeneticvariabili
7、tyandstructureofthespecies.ThemoleculartechniqueofThegiantredshrimp,Aristaeomorphafoliacea(RissoISSRsreliesontheampli?cationofDNAfragments1827),isanimportanteconomicresourceexploitedinthebetweentwocloselyandinverselyorientedmicrosatellitesMediterraneanSeasince1959[1].Recently,an
8、ew?shing[8].ISSRsdonotrequireanypreviousknowled