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1、雷公藤多甙對急性移植物抗宿主病小鼠的作用論文于艷秋，張義俠，金玉楠，盧曉梅，王巍，張海鵬【摘要】本研究應(yīng)用雷公藤多甙防治小鼠急性移植物抗宿主病。對受致死量照射的C57BL/6受鼠，注入供鼠BABC/c骨髓與脾淋巴細(xì)胞的混合液，并給予雷公藤多甙、環(huán)孢素A、甲氨蝶呤處理。用免疫組織化學(xué)法、流式細(xì)胞術(shù)和ELISA法檢測各組小鼠T淋巴細(xì)胞、黏附分子、細(xì)胞因子。結(jié)果表明：所有異基因?qū)φ战M小鼠.freelRNA的表達（P0.05）；上調(diào)血清中IL-10的水平（P0.05），但對IL-4的作用不顯著（P0.05）。結(jié)論：雷公藤多甙具有明顯抗aGVHD作用并
2、同時保留抗白血病效應(yīng)，其作用機制與調(diào)節(jié)促炎/抑炎細(xì)胞因子的分泌和黏附分子的表達，以及抑制T淋巴細(xì)胞作用有關(guān)?！娟P(guān)鍵詞】雷公藤多甙；移植物抗宿主?。籘淋巴細(xì)胞；黏附分子；細(xì)胞因子GlucosidorumTripterygiiTororum(GTT)isakindofChinesetraditionalmedicine(CTM),forthefirsttimeusedinChinaasanimmunoregulatoryagententofautoimmunologicaldiseasessuchasrheumatoidarthritis(RA)
3、andnephroticsyndrome.Ourpreviousexperimentshophocytes,inhibitthesecretionofIL-2andexpressionofIL-2R,doolecules(CD11a,CD18)onthesurfaceofTcells.Anditcanprolongthesurvivaltimeofallogeneictransplantedskin.Itseffectonanti-rejectionisethylprednisolone.And,.freelshouchmorestronge
4、reffect,indicatingthatGTTcanmoderatelyorobviouslyinhibitthefunctionofTcellsandthesecretionofcytokines［1］.Furthermore,GTTmayhaveanti-inflammatoryandanti-tumoreffects.Graft-Versus-Hostdisease(GVHD),especiallyacuteGraft-Versus-Hostdisease(aGVHD)isamajorlethalplicationafterbone
5、marroedtoadoptGTTorbineGTTechanism.MaterialsandMethodsMiceDonormiceBALB/c(H-2d),female;syngeneictransplantationrecipientmiceBALB/c(H-2d),male;allogeneictransplantationrecipientmiceC57BL/6(H-2b)male.AllofthemyofSciences).InductionofaGVHDTherecipientsreceivedin,SIMENZ,Primus,
6、Germany)for6-8hoursbeforetransplantation.BonemarroaleBALB/c(H-2d)edium.BothBALB/candC57BL/6recipientsarrophocytesviacaudalvein.AssignmentandprocessAftertransplantation,therecipientslydividedintofollo.Theirfoodandicin(320mg/L)anderythromycin(250mg/L)inistratedl;inCsA+MTXgrou
7、pg/kgeverydayandMTX7mg/m2ontheday1,3,5and9;inGTTgroupg/kg;inGTT+CsAgroupg/kgandCsA2.5mg/kg.GTTg/mlbyTiceesptomsofaGVHD(iceofallo-BMTgrouphadthesignificantclinicalsymptomsofaGVHD,suchasiceofsyn-BMTgrouphadnot.TherecipientmiceofGTT,GTT+CsAandCsA+MTXgroupsshoore,inappetencecou
8、ldbeobservedinGTTandGTT+CsAgroup.Onday14afterBMT,thetissuesamplesofskin,lungandint
