資源描述:
《新型重組高效復合干擾素對卵巢癌細胞生長抑制及促凋亡作用》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在學術(shù)論文-天天文庫。
1、新型重組高效復合干擾素對卵巢癌細胞生長抑制及促凋亡作用【摘要】目的:探討新型重組高效復合干擾素對卵巢癌HO8019細胞的作用,并與同類型復合干擾素、普通重組Ⅰ型干擾素、化療藥物對比。方法:應(yīng)用新型重組高效復合干擾素、復合α干擾素、干擾素α-2b和順鉑以0.156ug/ml、0.625ug/ml、2.5ug/ml及10ug/ml濃度作用于體外培養(yǎng)的HO8019細胞72小時后,MTT法檢測并計算細胞抑制率并比較。應(yīng)用新型重組高效復合干擾素、復合α干擾素、干擾素α-2b和順鉑以0.156ug/ml、0.625ug/ml、2.5ug/ml濃度作用于體外培養(yǎng)的HO8
2、019細胞72小時后,以流式細胞儀檢測HO8019細胞的凋亡率并進行比較。結(jié)果:幾種干擾素及順鉑均能導致HO8019細胞生長抑制及促進細胞凋亡。相同濃度條件下,新型重組高效復合干擾素和順鉑對HO8019細胞的抑制及凋亡作用強于復合α干擾素和干擾素α-2b的作用,尤以順鉑的作用最強。結(jié)論:重組高效復合干擾素對體外培養(yǎng)的卵巢癌HO8019細胞的生長有抑制作用,并能促進HO8019細胞凋亡;作用雖然不及化療藥物順鉑,但較同類型干擾素、普通重組Ⅰ型干擾素的作用強?!娟P(guān)鍵詞】干擾素;卵巢癌;基因重組TheGrowthInhibitionand10Αpoptosis-
3、promotingEffectOfrSIFN-cototheOvarianCarcinomaCellsinVitroTANGXiumei,HEKai,GAODehong,LIUBoTheFifthAffiliatedHospitalofQiaiHaerMedicalCollege,Heilongjiang163454,China【Abstract】Objectives:ToinvestigatetheeffectofrSIFN-coconsensusInterferon,IFNa-2b(Interferona-2b)andchemotherapeutics
4、onovariancancercelllineHO8019invitro.Methods:ΑfterHO8019cervicαlcαncercellswereculturedinvitrowithrSIFN-co、Infergen、IFNα-2bαndcisplαtin(DDP)αttheconcentrαtionof0.156ug/ml、0.625pg/ml、2.5ug/mland10g/mlrespectivelyfor72hours,cellproliferαtionwasmensuratedbyMTTassay.Cellinhibitionrate
5、wascalculated.ThenafterHO8019ovariancancercellsweretreatedwithrSIFN-co、Infergen、IFNa-2bandDDPattheconcentrationof0.156ug/ml、0.625pg/ml、2.5pg/mlfor72hours,cellapoptosiswαsdeterminedbyflowcytometry.Results:rSIFN-co、Infergen、IFNα-2bandDDPcouldinduceinhibitionandapoptosisofHO8019cells
6、.TheinhibitionandapoptosisofHO8019cells10whichhadbeeninducedbyrSIFN-coandDDPwerebetterthanwhichhadbeeninducedbyInfergenandlFNα-2batthesameconcentration,andDDPwasmorebetterthanrSIFN-co.Coclusions:rSIFN-cohasantineoplasticefectbyinhibitingproliferationandinducingapoptosisinHO8019cer
7、vicacancercellinvitro.TheeffectisinferiortoDDP,butbetterthanthesametypeinterferonandgeneraltypeIinterferon.【Keywords】Interferon;CervicaIcancer;Generecombination干擾素(interferon,IFN)是較早發(fā)現(xiàn)和應(yīng)用的一類細胞因子,不僅具有抗病毒復制、抑制細胞分裂、誘導細胞分化、增強細胞吞噬功能等作用,還可以調(diào)節(jié)免疫反應(yīng),誘導細胞特定基因的表達,加強機體在抗腫瘤方面的免疫應(yīng)答[1-3]。其中以a-干擾
8、素的抗腫瘤效果為優(yōu)。重組復合高效干擾素(recombinantsu