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《新型重組高效復(fù)合干擾素對(duì)卵巢癌細(xì)胞生長(zhǎng)抑制及促凋亡作用》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、新型重組高效復(fù)合干擾素對(duì)卵巢癌細(xì)胞生長(zhǎng)抑制及促凋亡作用【摘要】目的:探討新型重組高效復(fù)合干擾素對(duì)卵巢癌HO8019細(xì)胞的作用，并與同類型復(fù)合干擾素、普通重組Ⅰ型干擾素、化療藥物對(duì)比。方法：應(yīng)用新型重組高效復(fù)合干擾素、復(fù)合α干擾素、干擾素α-2b和順鉑以0.156ug/ml、0.625ug/ml、2.5ug/ml及10ug／ml濃度作用于體外培養(yǎng)的HO8019細(xì)胞72小時(shí)后，MTT法檢測(cè)并計(jì)算細(xì)胞抑制率并比較。應(yīng)用新型重組高效復(fù)合干擾素、復(fù)合α干擾素、干擾素α-2b和順鉑以0.156ug/ml、0.625ug/ml、2.5ug/ml濃度作用于體外培養(yǎng)的HO8
2、019細(xì)胞72小時(shí)后，以流式細(xì)胞儀檢測(cè)HO8019細(xì)胞的凋亡率并進(jìn)行比較。結(jié)果：幾種干擾素及順鉑均能導(dǎo)致HO8019細(xì)胞生長(zhǎng)抑制及促進(jìn)細(xì)胞凋亡。相同濃度條件下，新型重組高效復(fù)合干擾素和順鉑對(duì)HO8019細(xì)胞的抑制及凋亡作用強(qiáng)于復(fù)合α干擾素和干擾素α-2b的作用，尤以順鉑的作用最強(qiáng)。結(jié)論：重組高效復(fù)合干擾素對(duì)體外培養(yǎng)的卵巢癌HO8019細(xì)胞的生長(zhǎng)有抑制作用，并能促進(jìn)HO8019細(xì)胞凋亡；作用雖然不及化療藥物順鉑，但較同類型干擾素、普通重組Ⅰ型干擾素的作用強(qiáng)?！娟P(guān)鍵詞】干擾素；卵巢癌；基因重組TheGrowthInhibitionand10Αpoptosis-
3、promotingEffectOfrSIFN-cototheOvarianCarcinomaCellsinVitroTANGXiumei,HEKai,GAODehong,LIUBoTheFifthAffiliatedHospitalofQiaiHaerMedicalCollege,Heilongjiang163454,China【Abstract】Objectives:ToinvestigatetheeffectofrSIFN-coconsensusInterferon，IFNa-2b(Interferona-2b)andchemotherapeutics
4、onovariancancercelllineHO8019invitro．Methods：ΑfterHO8019cervicαlcαncercellswereculturedinvitrowithrSIFN-co、Infergen、IFNα-2bαndcisplαtin(DDP)αttheconcentrαtionof0.156ug／ml、0.625pg/ml、2.5ug/mland10g/mlrespectivelyfor72hours，cellproliferαtionwasmensuratedbyMTTassay.Cellinhibitionrate
5、wascalculated.ThenafterHO8019ovariancancercellsweretreatedwithrSIFN-co、Infergen、IFNa-2bandDDPattheconcentrationof0.156ug/ml、0.625pg/ml、2.5pg/mlfor72hours，cellapoptosiswαsdeterminedbyflowcytometry．Results：rSIFN-co、Infergen、IFNα-2bandDDPcouldinduceinhibitionandapoptosisofHO8019cells
6、.TheinhibitionandapoptosisofHO8019cells10whichhadbeeninducedbyrSIFN-coandDDPwerebetterthanwhichhadbeeninducedbyInfergenandlFNα-2batthesameconcentration，andDDPwasmorebetterthanrSIFN-co.Coclusions：rSIFN-cohasantineoplasticefectbyinhibitingproliferationandinducingapoptosisinHO8019cer
7、vicacancercellinvitro．TheeffectisinferiortoDDP，butbetterthanthesametypeinterferonandgeneraltypeIinterferon．【Keywords】Interferon；CervicaIcancer；Generecombination干擾素(interferon，IFN)是較早發(fā)現(xiàn)和應(yīng)用的一類細(xì)胞因子，不僅具有抗病毒復(fù)制、抑制細(xì)胞分裂、誘導(dǎo)細(xì)胞分化、增強(qiáng)細(xì)胞吞噬功能等作用，還可以調(diào)節(jié)免疫反應(yīng)，誘導(dǎo)細(xì)胞特定基因的表達(dá)，加強(qiáng)機(jī)體在抗腫瘤方面的免疫應(yīng)答[1-3]。其中以a-干擾
8、素的抗腫瘤效果為優(yōu)。重組復(fù)合高效干擾素(recombinantsu
