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1、第六卷第四期銅仁職業(yè)技術(shù)學(xué)院學(xué)報(bào)(自然科學(xué)版)Vol.6.No.42008年8月JournalofTongrenVocational&TechnicalCollege(NaturalScienceEdition)Aug.2008山羊抗小鵝瘟高免血清的制備及應(yīng)用王文強(qiáng)122,王文成,熊鳳蘭(1.銅仁職業(yè)技術(shù)學(xué)院生物工程系,貴州銅仁554300;2.銅仁市畜牧獸醫(yī)局,貴州銅仁554300)摘要:小鵝瘟是雛鵝感染鵝細(xì)小病毒(Gooseplaguevirus,GPV)所引起的一種急性敗血型傳染病,病程短且
2、死亡率很高,主要侵害1月齡以內(nèi)的雛鵝和雛番鴨。目前,市場銷售的對此病進(jìn)行免疫和治療的抗小鵝瘟血清存在產(chǎn)量低、成本高,且容易導(dǎo)致某些鵝源疫病的傳播的缺點(diǎn)。筆者采用從貴州銅仁市等地的養(yǎng)鵝場采集的病鵝分離出的小鵝瘟野生強(qiáng)毒株接種于9日齡的鵝胚尿囊液制備了小鵝瘟病毒抗原,并用小鵝瘟病毒抗原加弗氏佐劑后,分多次皮下注射結(jié)合靜脈注射小鵝瘟病毒抗原免疫貴州黑、白山羊。頸動脈一次性放血制備抗小鵝瘟異源高免血清,用瓊脂擴(kuò)散進(jìn)行效價(jià)的測定,結(jié)果顯示其抗體效價(jià)平均都在1∶8以上。將制備的血清與市售的抗小鵝瘟進(jìn)行了對比試
3、驗(yàn),結(jié)果市售的抗小鵝瘟血清的保護(hù)率為85%,山羊抗小鵝瘟高免血清的保護(hù)率為95%。山羊抗小鵝瘟異源高免血清具有效價(jià)高、成本低、安全性好等優(yōu)點(diǎn)。關(guān)鍵詞:小鵝瘟;山羊抗小鵝瘟高免血清;抗小鵝瘟血清;對比試驗(yàn)中圖分類號:S854.5+7文獻(xiàn)標(biāo)識碼:B文章編號:310-(2008)04-0001-(06)StudyandApplicationofHigh-ImmunizedSerumfromGoatAgainstGoslingPlague122WANGWen-qiang,WANGWen-cheng,XIO
4、NGFeng-lan(1.DepartmentofBiology&Engineering,TongrenVocational&TechnicalCollege,Tongren554300,Guizhou;2.BureauofAnimalHusbandryofTongrenCity,Tongren554300,Guizhou)Abstract:GoosePlague(GP),anacuteinfectiousdiseaselostbloodtypeingooes,wascausedbygoosepa
5、rvovirus(GPV),Shortcourseofthediseaseandhighmortality,mainlyagainstthelessthan1montholdandyounggoslingsMuscovy.Followingthe10-day-oldgoslingsmorbidity,mortalityratecouldbeashighas100percent.Atpresent,thatanti-plagueserumonGooseinmarketwasusedinmanyGoo
6、sefarmwasimmunizedandtreatedtoGosling.However,itislow-yield,highcost,andeasilyleadtothediseaseofgeesesourcespread..Inthispaper,thewildgoslingplagueXWhighlyvirulentstrainsofvaccinationthatwasisolatedfromTongren,GuizhouandotherplacesGoosefarm,.goslingpl
7、aguevirusantigenwaspreparedinthe9-day-oldembryogooseallantoicfluidwiththeVirus,gooseparvovirusantigenaddedwithFreund,scompleteAdjuvantwasusedtoimmunizedgoatsofguizhouwithseveralspotssubcutaneousandvieninjection.serumofanti-alienGoosePlagueHigh-immuniz
8、edwascollectedinCarotidarterybleedingofone-time.VirulentGooseparvovirusantigenwasusedtoimmunizedgoatsintravenousinjection.High-immunizedserumwith1:8Agarprecipitationantibodytiterswasgetted.Willbepreparedbytheanti-serumandthemarketblastwerecomp
