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1、精漿游離MicroRNA34―b在特發(fā)性少精子癥及無精子癥中的表達(dá)和臨床應(yīng)用 [摘要]目的研究游離MicroRNA34-b(miR-34b)在特發(fā)性少精子癥和特發(fā)性無精子癥患者精漿中的表達(dá)及診斷價值。方法收集特發(fā)性少精子癥、特發(fā)性無精子癥和同期因女方因素不孕前來檢查的生育力評估正常的健康男性精液各20份,采用實時熒光定量PCR檢測精漿游離miR-34b的相對含量,通過方差分析統(tǒng)計各組間的差異,并對精漿游離miR-34b在特發(fā)性少精子癥和特發(fā)性無精子癥中的診斷價值進(jìn)行ROC曲線分析。結(jié)果正常對照組、特發(fā)性少精子癥組和特發(fā)性無精子癥組三組間精漿miR-34b表達(dá)
2、量差異有高度統(tǒng)計學(xué)意義(F=21.44,P<0.01),與正常對照組相比,特發(fā)性少精子癥組和特發(fā)性無精子癥組精漿miR-34b表達(dá)量顯著降低,差異有高度統(tǒng)計學(xué)意義(P均<0.01),但特發(fā)性少精子癥組與特發(fā)性無精子癥組間精漿miR-34b相對含量差異無統(tǒng)計學(xué)意義(P=0.31)。ROC曲線顯示miR-34能夠較好的區(qū)分特發(fā)性少精子癥組與健康對照組以及特發(fā)性無精子癥組與健康對照組曲線下面積分別為(AUC)為0.85(95%CI,0.73~0.97)、0.90(95%CI,0.80~0.99)。結(jié)論miR-34b在特發(fā)性少精子癥和特發(fā)性無精子癥患者精漿中明顯降低,
3、并對特發(fā)性少精子癥和特發(fā)性無精癥子癥顯示出一定的診斷價值?! 關(guān)鍵詞]特發(fā)性少精子癥;特發(fā)性無精子癥;miR-34b;精漿 []R698.2[]A[]1673-9701(2015)25-0004-04 TheexpressionandclinicalroleofseminalplasmafreeMicroRNA34-binpatientswithidiopathicoligospermiaandazoospermia LOUJiangtaoWEIRenxiongYULiangliangSHIBoCUIYun MaleDivisionLaborator
4、y,NingboTraditionalChineseMedicineHospitalAffiliatedtoZhejiangChineseMedicineUniversity,Ningbo315012,China [Abstract]ObjectiveToquantifytheexpressionofseminalplasmafreemiR-34binpatientswithidiopathicoligospermiaandazoospermiaandtoanalyzethediagnosticvalueofit.Methods20casessemenofI
5、diopathicoligospermiaandazoospermiawerecollected,respectively.Atthesametime,20casessemenofhealthymalewithfertilityevaluationwhichbecauseofthewomanfactorleadtoinfertilitywerecollectedascontrolgroup.therelativeexpressionofseminalplasmafreemiR-34bwasdetectedbyreal-timefluorescentquanti
6、tativePCR,thedateswereanalyzedbyvarianceanalysis,thediagnosticvalueofseminalplasmafreemiR-34bindiseasesofoligospermiaandazoospermiawereanalyzedbyROCcurve.ResultsTherelativeexpressionofseminalplasmafreemiR-34binoligospermiagroupandazoospermiagroupweredecreasedcomparedwithnormalcontro
7、ls(P<0.01),whiletherewerenotchangebetweenoligospermiagroupandazoospermiagroup(P=0.31).ROCcurveshowedthatmiR-34bcoulddistinguishbetweenoligospermiadiseaseandhealthycontrol,theazoospermiaandhealthycontrol,theareaunderthecurvewere0.85(95%CI,0.73-0.97)and0.90(95%CI,0.80-0.99),respective
8、ly.ConclusionTheexp