11例急性巨核系白血病患者巨核細(xì)胞的超微結(jié)構(gòu)分析

《11例急性巨核系白血病患者巨核細(xì)胞的超微結(jié)構(gòu)分析》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、11例急性巨核系白血病患者巨核細(xì)胞的超微結(jié)構(gòu)分析作者：茹永新，趙軾軒，劉津華，秘營昌，竺曉凡，王慧君，王建祥【摘要】為了研究急性巨核細(xì)胞白血病患者骨髓中幼稚巨核細(xì)胞的超微結(jié)構(gòu)特點(diǎn)，根據(jù)電子顯微鏡觀察結(jié)果，回顧性分析11例急性巨核白血病患者骨髓巨核細(xì)胞的形態(tài)結(jié)構(gòu)和血小板過氧化物酶（PPO）表達(dá)情況。結(jié)果表明：11例中7例例巨核細(xì)胞直徑在20μm以內(nèi)，其中2例細(xì)胞直徑10-15μm，PPO陽性率50%以上，大部分細(xì)胞外形規(guī)則，大小均勻，表面突起少，核不規(guī)則，核質(zhì)比大，胞漿顆粒細(xì)小，分界膜發(fā)育不完全，管道排列無規(guī)律；5例巨核細(xì)胞直徑20μm左右，PPO陽性率在8%-22%之間，核呈圓形

2、或馬蹄形，異染色質(zhì)不等，其中3例無幼稚分界膜和致密顆粒，2例偶見。11例中5例細(xì)胞直徑在20-40μm之間，PPO陽性率16%-80%，大細(xì)胞表面有不規(guī)則突起，核質(zhì)比不等，核不規(guī)則，異染色質(zhì)多，核仁不明顯，胞質(zhì)有成熟分界膜結(jié)構(gòu)、管道系統(tǒng)和成熟α顆粒。結(jié)論：大部分患者巨核細(xì)胞以Ⅰ期分化階段為主，少數(shù)病例伴有少量Ⅱ、Ⅲ期細(xì)胞；相同或不同患者骨髓中未成熟巨核細(xì)胞的發(fā)育程度存在差異。【關(guān)鍵詞】急性巨核細(xì)胞白血病10UltrastructuralCharacteristicsofMegakaryocytesin11PatientswithAcuteMegakaryocyticLeukem

3、iaAbstractThepurposeofstudywastoinvestigatetheultrastructuralfeaturesofleukemicmegakarocyte(LMK)inpatientswithacutemegakaryocyticleukemia(M7).AnalyzingtheultrastructurecharacteristicsofLMKandpositiveratioofplateletperoxides(PPO)in11patientswithM7wereanalyzedonbasisoftransmissionelectronmicros

4、copicobservationretrospectively.TheresultsshowedthatthediameterofLMKin7outof11caseswaslessthan20μm,in2casesofthem,theLMKdiameterwasfrom10to15μmandtheirPPOpositiveratiowasmorethan50%,mostLMKdisplayedregularshape,lessprotrusions,irregularnucleus,highnuclear/cytoplasmratio,tinygranules,undevelop

5、eddemarcationmembranesystem(DMS)andirregulartubulesincytoplasm;in5outofthose7casesthediameterofLMKwasabout20μm,PPOpositivecellcountwasfrom8%to22%,mostshowingroundorhorseshoenuclei,moreorlessheterochromatin,noDMSandgranuleswerefoundinLMKin3casesand2casesoccasionally.Inother5outof11cases,thedia

6、meterofLMKwasfrom20to40μmandPPOpositiveratio10wasfrom16%to80%,inwhichsmallerLMKsweresimilartothoseinformercasesinshape,andthelargerLMKhadirregularprotrusions,variednuclear/cytoplasmratio,moreheterochromatin,prominentnucleolus,someofthemcontaineddevelopedDMS,tubulesandαgranules.Itisconcludedt

7、hatmostpatientswithM7arepredominantofLMKinstageIandminoritycontainedLMKinIIorIIIstagesimultaneously.ThedifferentiationdegreesofLMKaredifferentinindividualandvariouscases.Keywordsultrastructure;acutemegakaryocyticleukemia;differentiationJExpH