大蔥油對胃癌細胞株體外培養(yǎng)的影響

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1、大蔥油對胃癌細胞株體外培養(yǎng)的影響【摘要】　　目的：探討大蔥油對胃癌細胞的影響以及誘導胃癌細胞凋亡的機制.方法：MTT法評價大蔥油對胃癌細胞增殖的影響.熒光染色觀察細胞形態(tài)學變化.流式細胞術和放免法檢測細胞凋亡及細胞內游離Ca2+和環(huán)磷酸腺苷（cAMP）濃度.結果：隨著大蔥油濃度的增加，作用24h后，細胞增殖抑制率分別達到5.71%，36.33%，44.34%，54.52%和74.15%，IC50≈65μg/mL.熒光顯微鏡下觀察結果顯示，實驗組出現細胞凋亡形態(tài)學變化.100μg/mL大蔥油作用于胃癌細胞1~12h，細胞

2、總凋亡率分別為50.16%，53.48%，77.84%，83.71%和41.34%；早期細胞凋亡率分別為50.15%,33.25%,11.69%，4.65%和0.84%.100μg/mL大蔥油作用于胃癌細胞5min~12h，各相應時間點細胞內游離Ca2+含量依次為(71.00±6.08)，(77.67±1.53),(79.67±1.53)，(83.33±5.03)，(76.00±6.06)，(75.67±2.52)，(72.33±4.24)和(65.00±2.00)nmol/L；細胞內cAMP濃度依次為(5.76±0.

3、36),(18.51±2.06)，(20.40±1.65)，(16.09±1.46)，(12.16±0.78)，(9.88±0.53)，(9.47±0.78)和(9.82±1.06)pmol/mg（P<0.05).結論：大蔥油可抑制胃癌細胞增殖，并通過上調細胞內Ca2+和cAMP水平誘導其凋亡.【關鍵詞】大蔥油；胃腫瘤；腫瘤細胞，培養(yǎng)的；凋亡；Ca2+；cAMP12　　EffectsofAlliumfistulosumL.oiloninvitrocultureofgastriccancercellline　　【A

4、bstract】AIM:ToinvestigatetheeffectsofAlliumfistulosumL.oil(ALFO)ongastriccancercelllineandthepossiblesignalmolecularmechanismunderlyingapoptoticprocessinducedbyAFLO.METHODS:HumangastriccancerMGC803cellswereculturedinvitroandtreatedbyAFLO.Thenitsgrowthinhibitory

5、ratewascountedandIC50wasmeasured.Apoptoticprocesswasinvestigatedwithfluorescencemicroscope.Intracellularfreecalciumcontentatdifferenttimeswasdeterminedbyflowcytometry.Intracellularcyclicadenosinemonophosphate(cAMP)levelsweredeterminedbyradioimmunoassay(RIA).RE

6、SULTS:WithincreasingtheconcentrationofAFLO,cellgrowthinhibitoryrateswentupcorrespondingly24haftertreatmentwithAFLOandreached5.71%,36.33%,44.34%,54.52%and74.15%respectively.IC50≈65μg/mL.Hoechst33258fluorescentstainingshowedthatapoptosisappearedinsomecellstreatedb

7、yAFLO(100μg/mL).WhenMGC803cellswereincubatedinthepresenceofAFLO(100μg/mL)from1to12h,thetotalapoptoticrateswere50.16%,53.48%,77.84%,83.71%and41.34%,respectively,andtheearlyapoptoticrateswere1250.15%,33.25%,11.69%,4.65%and0.84%,respectively.InMGC803cellstreated

8、byAFLO(100μg/mL)for5minto12h,freecalciumcontentswere(71.00±6.08),(77.67±1.53),(79.67±1.53),(83.33±5.03),(76.00±6.06),(75.67±2.52)，(72.33±4.24)and(65.00±2.00)nmol/L,re