資源描述：
《抗原沖擊致敏樹突狀細(xì)胞誘導(dǎo)特異性ctl殺傷jurkat細(xì)胞實驗研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、抗原沖擊致敏樹突狀細(xì)胞誘導(dǎo)特異性CTL殺傷Jurkat細(xì)胞實驗研究[字體：大中小]作者：林東軍，方志剛，李旭東，劉加軍，陸英【摘要】 本研究以健康人外周血單核細(xì)胞為前體細(xì)胞，體外誘導(dǎo)其為樹突狀細(xì)胞（DC），分別負(fù)載Jurkat細(xì)胞凍融抗原及WT1多肽抗原，產(chǎn)生特異性細(xì)胞毒性T淋巴細(xì)胞（CTL），以探討其對白血病Jurkat細(xì)胞的殺傷作用。聯(lián)合應(yīng)用rhGM-CSF、rhIL-4、rhTNF-α及rhsCD40L等細(xì)胞因子，密度梯度離心法、貼壁法從外周血獲取單核細(xì)胞并進(jìn)行誘導(dǎo)擴(kuò)增，培養(yǎng)出DC，分別用凍融抗原及WT1多肽抗原沖擊致敏DC。實驗分4組：凍融抗原致敏
2、DC組為實驗組A，WT1多肽致敏DC組為實驗組B，未致敏DC組為對照組A，單核細(xì)胞組為對照組B，觀察CTL對Jurkat細(xì)胞的殺傷作用。結(jié)果表明：培養(yǎng)出了具有典型特征的DC，它高表達(dá)CD40、CD80、CD1a及CD86等表面標(biāo)志，能體外誘導(dǎo)強(qiáng)烈的同種異基因混合淋巴細(xì)胞增殖反應(yīng)。實驗組顯示高水平殺傷率，與對照組比較均具有統(tǒng)計學(xué)意義（P＜0.01），實驗組A、B間比較無統(tǒng)計學(xué)意義。結(jié)論：Jurkat細(xì)胞凍融抗原及WT1多肽抗原沖擊致敏DC能有效誘導(dǎo)T細(xì)胞抗白血病作用，為臨床研制DC疫苗提供了實驗依據(jù)?！娟P(guān)鍵詞】樹突狀細(xì)胞；Jurkat細(xì)胞；凍融抗原；WT1多肽
3、抗原；細(xì)胞毒作用；白血病 Antigen-loadedDendriticCellsTriggerKillingEffectsofSpecificCyto-toxicTLymphocytesonJurkatCellsInVitro AbstractThisstudywasaimedtoinvestigatetheeffectsoftumorantigen-loadeddendriticcells(DC)stimulatingthespecificcytotoxicTlymphocytes(CTL)onJurkatcellsinvitro.Periphera
4、lbloodmononuclearcellswereisolatedbyFicolldensitygradientcentrifugationfromnormalhumanheparinizedblood，theadherentmonocyteswereculturedwithgranulocyte-macrophagecolonystimulatingfactor(GM-CSF)，interleukin-4(IL-4)，alphatumornecrosisfactor(TNF-α)andsCD40L，DCswereco-culturedwithfrozen
5、-thawedantigenofJurkatcellsorWT1peptides，andthenTcellsweretriggeredintospecificCTL.TheresultsshowedthatmostsuspendedcellsexhibiteddistinctivemorphologicalfeaturesofDCwhichexpressedCD40(96%)，CD86(97%)，CD80(77%)，CD1a(69%)，andgainedthepowerfulcapacitytostimulateproliferationofallogenE
6、iclymphocytes.Undertheeffector∶targetratioof20∶1，CTLsderivedfromcultureswithDCandfrozen-thawedantigenofJurkatcellsshowed91.1%cytotoxicityagainstJurkatcells，CTLderivedfromcultureswithDCandWT1peptidesshowed87.5%cytotoxicityagainstJurkatcells，cytotoxicityofCTLderivedfromcultureswithun
7、loadedDCagainstJurkatcellswas42.1%andcytotoxicityofmonocyteswas22.7%.CytotoxicityofCTLderivedfromculturewithfrozen-thawedantigenorWT1peptidesloadedDCwasstrongerthanthatincontrolgroups（P＜0.01）.Itisconcludedthatthetumorantigen-pulsedDCcaninduceefficientandspecificanti-tumorimmunity，m
8、ayplayagreatroleinclinical
