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1、中國(guó)協(xié)和醫(yī)科大學(xué);中國(guó)醫(yī)學(xué)科博士學(xué)位論文異型M-CSF/M-CSFRDNA疫苗構(gòu)建及其免疫姓名：王敏慧申請(qǐng)學(xué)位級(jí)別：博士專(zhuān)業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：吳克復(fù);宋玉華2002.5.1縮寫(xiě)詞表綾喳堡苤塞全登主塞鎏登AAbsorbency吸光值A(chǔ)BCAvidin—biotincomplex卵白素生物素復(fù)合物APCAntigenpresentingcells抗原遞呈細(xì)胞ALAcuteleukemia急性白血病BMMCBonemarrowmononuclearcell骨髓單個(gè)核細(xì)胞bpBasepair堿基對(duì)BSABovineserumalbumin牛血清白蛋白CDClusterdet
2、ermination分化抗原CEACarcinoembryonicantigen癌胚抗原CSF一1Colony—stimulatingfactor-1集落刺激因子一1CTLCytotoxicTlymphocyte細(xì)胞毒T淋巴細(xì)胞dDay天DABDiaminobenzidine二氨基聯(lián)苯胺DCDendriticcells樹(shù)突狀細(xì)胞DMSODimethylsulfoxide二甲基亞砜DTTDithiothreit01二硫蘇糖醇EBEthidiumbromide溴化乙錠EDTAEthylenediaminetetraaceticacid乙二胺四乙酸ELlSAEnzyme．1i
3、nkedimmuno—酶聯(lián)免疫吸附測(cè)定absorbenceassayG418Geneticin慶大霉素418GAPDHGlyceraldehyde3一phosphate甘油醛三磷酸脫氫酶dehydrogenasehHour小時(shí)HIVHumanimmunodeficiencyvirus人類(lèi)免疫缺陷病毒HHVHumanherpesvirus人類(lèi)皰疹病毒IFN—YInterferon—gammaY一干擾素ILInterleukin白細(xì)胞介素IPTGIsopropylthio·§一D—galactoside異丙基硫代～B—D一半乳糖苷酶ISS免疫激活DNA序列KbKilobas
4、e-pairs千堿基對(duì)KDaKilodalton千道爾頓KSKaposi’ssarcoma卡波氏肉瘤LBLuria，BertanimediumLB培養(yǎng)基MAbMonoclonalantibody單克隆抗體MCDMulticentricCastleman’sdisease多中心性Castleman氏病MCSMulti-cloningsite多克隆位點(diǎn)MAFJ6．1Membrane-associated膜相關(guān)性生長(zhǎng)因子growthfactorMAFJ6．1RMembralle—associatedgrowth膜相關(guān)性生長(zhǎng)因子受體factorreceptorM．CSFMacr
5、ophagecolony—stimulating巨噬細(xì)胞集落刺激因子factorM．CSFRMacrophagecolony-stimulating巨噬細(xì)胞集落刺激facmrrecepmr因子受體mgMilligram毫克mlnMinute分鐘m．M—CSFMembrane．．boundM．．CSF膜結(jié)合型M．CSFNSNormalsaline生理鹽水ODOpticaldensity光密度值OPDO—Phenylenediamine鄰苯二胺二氫氯dihydrochloridePBMCPeripheralbloodmononuclearcell外周血單個(gè)核細(xì)胞iiPCRP
6、olymemsechainreaction聚合酶鏈反應(yīng)PELPimaryeffusionlymphoma原發(fā)性滲出性淋巴瘤PG．M—CSFProteoglycan—boundM-CSF細(xì)胞外基質(zhì)結(jié)合型pFM．CSFR-m-M．-CSF融合DNA疫苗pMm．M．CSFDNA疫苗pRM—CSFRDNA疫苗rpmRotateperminute每分鐘轉(zhuǎn)速RT—PCRRevel'setranscription—PCR逆轉(zhuǎn)錄一PCRsecSecond秒SDSSodiumdodecylsulfate十二烷基硫酸鈉SDS．PAGESDS-polyacrylamidgelSDS．聚丙烯酰
7、胺凝膠electrophoreses電泳s．M．CSFSolubleM．CSF可溶型M-CSFSP2／0一F穩(wěn)定表達(dá)人M—CSFR—m．M．CSF的小鼠SP2／0細(xì)胞系弘ATumorassociatedantigen腫瘤相關(guān)抗原ThThelperlymphocyte輔助性T細(xì)胞Tris3-hydroxymethylaminomethane三羥甲基氨基甲烷中國(guó)醫(yī)學(xué)科學(xué)院中國(guó)協(xié)和醫(yī)科大學(xué)博士學(xué)位論文摘要巨!塞塑塑塞蓬型遨墾亍(M—CSF)是多功能的細(xì)胞因子，在單核_巨噬細(xì)胞的增殖、分化和妊娠、胚胎發(fā)育及骨骼形成等過(guò)程中發(fā)揮重要作用。由于選擇性
