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1、Osterix基因敲除及過(guò)表達(dá)對(duì)小鼠脊椎骨量的影響*陳思旭，宗兆文，賈敏，華祥，沈岳，趙玉峰，郭慶山，劉道城（400042重慶，第三軍醫(yī)大學(xué)大坪醫(yī)院野戰(zhàn)外科研究所創(chuàng)傷科，創(chuàng)傷、燒傷與復(fù)合傷國(guó)家重點(diǎn)實(shí)驗(yàn)室）[摘要]目的研究小鼠Osterix基因敲除及過(guò)表達(dá)后對(duì)其脊椎骨量的影響及作用機(jī)制。方法將12周齡的野生型小鼠(WT)、Osterix基因敲除小鼠(Osterix-KO)和Osterix過(guò)表達(dá)轉(zhuǎn)基因小鼠(Osterix-CA)分為3組（每組8只，均為雄性），分別采用X線(xiàn)攝像和HE染色觀察小鼠腰椎骨量的變化，TRAP染色檢測(cè)破骨細(xì)胞水平，免疫組織化學(xué)檢測(cè)NF-κB受體活化因子配體(RAN
2、KL)、骨涎蛋白(BSP)和骨橋蛋白(OPN)表達(dá)水平，并將所得數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果Osterix-KO小鼠在X線(xiàn)和HE染色表現(xiàn)為腰椎骨密度和骨量增加，破骨細(xì)胞數(shù)量顯著減少(P<0.05)，椎體中RANKL、BSP和OPN的表達(dá)水平顯著降低(P<0.05)，而Osterix-CA小鼠的脊椎X線(xiàn)、HE染色表現(xiàn)，及破骨細(xì)胞數(shù)量與WT小鼠無(wú)明顯差異。結(jié)論Osterix基因敲除小鼠脊柱骨吸收活性減弱伴成骨細(xì)胞分化能力降低，進(jìn)而發(fā)生骨量明顯增加。[關(guān)鍵詞]Osterix；脊椎；骨量[中圖法分類(lèi)號(hào)][文獻(xiàn)標(biāo)志碼]AEffectTheroleofOsterixosterixknock-outo
3、rover-expressionioncontrollingbonevolumeinvertebralbody.inmiceChenSixu,ZongZhaowen,JiaMin,HuaXiang,ShenYue,ZhaoYufeng,GuoQingshan,LiuDaocheng(StateKeyLaboratoryofTrauma，,BurnandCombinedInjury，,DepartmentofTrauma，,InstituteofSurgeryResearch，,DapingHospital，,ThirdMilitaryMedicalUniversity，,Chongq
4、ing，,400042，,China)[Abstract]ObjectiveToinvestigatethechangesofbonevolumeinvertebralbodyandtheunderlyingpossiblemechanisminOosterixknock-out(Osx-KO)andconstitutive-activation(Osx-CA)mice.MethodsAtotalof24Malemalemiceweredividedintothree3groups,thatis,includingwildtype(WT),Osx-KOandOsx-CAgroups(
5、n=8each).X–-rayradiologyandHEstainingwereusedtoevaluatethechangeofbonevolume.TRAPstainingwasusedtoassesstheactivityofosteoclasts.Immunohistochemistrycalassay(IHC)wasusedtoexaminetheexpressionlevelsofRANKL,BSPandOPN.TheIHCandTRAPstainingresultsweresemi-quantitiveanalyzedbymanualcountingmethod.Re
6、sultsX–-rayexaminationandHEstainingshowedthatthebonedensityandbonevolumeinthelumbarvertebralbodywasincreasedsignificantlyinOSX-KOmice,whileTRAPstainingshowedthatthenumberofosteoclastswasdecreased(P<0.01),andIHCrevealedthattheexpressionlevelsofRANKL,BSPandOPNweredown-regualted(P<0.01)inOSX-KOmic
7、e.However,Nnoobviouschangeswasfoundinofthebonedensity,bonevolumeandthenumberofosteoclastswerefoundinOsterix-KOmicecomparedwiththeWTmice.ConclusionsOsterixknock-outmayweakenboneresorptionactivityandosteoblastdifferentiationinthelum
