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《歸肝經(jīng)中藥對肝癌hepg2細胞增殖抑制與凋亡誘導作用.研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、ExperimentalstudyonthedominanceofproliferationinhibitionandapoptosisinducingofChineseherbswithliverchannel-tropisminHepG2ABSTRACTTutor:XiaoZhuMaster:HuangLizhongProfessorandTianXuefeiProfessor(HunanUniversityofTraditionalChineseMedicine,410208)Thehepatocarci
2、nomaoccupiesthesecondrateofcancerinChina.Atpresent,therewerenospecialdrugstotreatlate,midtrimesterandrecurrenthepatocarcinoma.TheChinesemedicinewasreceivedgreatattentionforimprovingclinicalsymptomsandlifequality,prolongingsurvivaltimesinclinicalanti-hepatoca
3、rcinomatherapy.InrecentyearstheChinesemedicinehadmadesomeprogressesinclinicalanti-hepatocarcinoma.However,itsmechanismofanti-hepatocarcinomawasstillnotcompletelydefined.Accordingly,researchingontheanti-hepatocarcinomamechanismofChinesemedicineandseekingtheef
4、fectivecomponentshadimportantsignificanceintheexplorationofnewtherapeuticmethods.Purpose:ToobservetheeffectofthetraditionalChinesemedicineascribingtothelivermeridianincellmultiplicationandapoptosisofHepG2in-vitroandinitiallytodiscussthetrendoftheanti-hepatoc
5、arcinomaeffecttotheChinesemedicineascribingtothelivermeridian.Methods:1.ZedorayRhizome,LeechandSalviaascribingtothelivermeridianwereselectedtocomparewithHedyoticdiffusaascribingtothenon-livermeridian.ZedoaryTurmericOilwasabstractedbyelaeo-volatilizedway,Salv
6、iaandHedyoticdiffusawaterextractwereabstractedbythemethodofaquafer-extrac＆alcohol-sinked.Leechwaterextractwasabstractedbythemethodofaquafer-extracted＆alcohol-sinkedandtacho-freeze-thawinginliquidnitrogen.SalviaandHedyoticdiffusawaterextractcontentwereidentif
7、iedbyHighperformanceliquidchromatography（HPLC）.ZedorayRhizomeOilwasassayedbyelaeo-volatilizedway.ThetwopreparationmethodsofLeechwaterextractwerecontrasted.2.UsingMTTassayedmultiplicationandinhibitionofZedorayRhizome,LeechandSalviaextractascribingtotheliverme
8、ridianonHepG2cellswhichcomparedwithHedyoticdiffusaextractascribingtothenon-livermeridianandblankcontrolgroup.Accordingtolinearregressionequationcalculatedthedrugconcentrationandcontentoftheextra
