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《il17a對(duì)gmcsf誘導(dǎo)的骨髓樹突狀細(xì)胞的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、天津醫(yī)科大學(xué)碩士學(xué)位論文costimulatorymoleculesCD40，CD86andMHCIIbyinductionGM．CSEAtthesametime．ithasadose．dependent．InordertoconfirmtheeffectofIL．17AtomllydifferentiatedBMDC，weaddedrmlL．17(50，100or500ng／m1)toBMDCinducedbyGM．CSFfor8days．thencontinueculture36hours．Compared、析tIlN0rmlL．17
2、A，BMDCsurfacecostimulatorymoleculesCD40，CD80，CD86，MHCIIandthecytokinessecretioninthesupernatantIL．12p40andIL．10werenotsignificantlychangedofeachgroup．ItshowsthatIL一17AhasnofunctionofstimulatingfullydifferentiatedBMDCtomature．ButIL．17Aup．regulatestheexpressionofcostimulato
3、rymoleculesonDCandthesecretionofIL．12P40andIL．1OintheprocessofGM．CSF．induced．BMDCwithLPS．Conclusions：T11iSexperimentusesthemethodofGM．CSF．inducingandLPSstimulizationsuccessfullyculturedandamplificationDC．IL．17ApromotesthedevelopmentofDCprogenitorsphenotypewhichisinducedby
4、GM-CSF．IL一17AalonehasliRleinfluenceonthedifferentiationofDC．IL．17Aup．regulatestheexpressionofDCcostimulatorymoleculesincludingCD40．CD80．CD86andMHC11whichaleexpressedonmatureDCstimulatedbyLPS．Italsoup—regulatethelevelofIL一12p40andIL一10secretedbyDCinducedbyLPS．Thesefindings
5、suggestthatIL一17AplaysanimportantroleinpromotingDCdifferentiation．Keywords：BMDCIL一17AGM—CSFDCcostimulatorymoleculeIL··12p40IL·-10IV天津醫(yī)科大學(xué)碩士學(xué)位論文目錄中文摘要???????????????????????????IAbstract·······················································································
6、II符號(hào)說(shuō)明???????????????????????????VI前言?????????????”????????????????·1月IJ舌?????????????”????????????????。l研究現(xiàn)狀、成果?????????????????????????2研究目的、方法??????????????????????????3對(duì)象和方法????????????????????????????41實(shí)驗(yàn)材料??????????????????????????41．1主要試劑??????????????????????????4
7、1．2主要儀器和設(shè)備???????????????????????51．3主要試劑的配制???????????????????????51．4實(shí)驗(yàn)動(dòng)物??????????????????????????72實(shí)驗(yàn)方法???????????????????????????72．1小鼠BMDC的分離和培養(yǎng)??????????????????72．2CDllc免疫磁珠分離提純成熟BMDC?????????????82．3流式細(xì)胞術(shù)檢測(cè)成熟BMDC表面分子?????????????82．4ELISA檢測(cè)細(xì)胞因子分泌?????????????????
8、?92．5統(tǒng)計(jì)分析????????????????????????10結(jié)果???????????????????????????????11l小鼠骨髓來(lái)源的DC體外擴(kuò)增方法的建立???????
