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《肽核酸靶向抑制線粒體融合逆轉(zhuǎn)人肺腺癌干細(xì)胞順鉑耐藥的分析》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、AbstractandWesternblottechniquerespectivelydetectthemRNAandproteinexpressionofMitofusin1,Mitofusin2,Opa1andAtad3ainA549cellspheresandA549cells.FlowcytometryinstrumentdetectmitochondriamembranepotentialofA549cellsandA549cellspheres;observingthedistributionofmitochondriainA549cellspheresa
2、ndA549cellsbyLaserConfocalMicroscop(LSCM);Solidphasepeptidesynthesispeptidenucleicacid-threebenzenephosphoruscompounds.LSCMobservatethetransfectionandidentificationofpeptidenucleicacid-threebenzenephosphoruscompoundsinlungcancerstemcells.RT-PCRandWesternblotdetectethemtDNAgeneexpression
3、lungcancerstemcellsandwesternblotdetectetheexpressionofmitochondrialfusionrelatedproteinMfn1,Mfn2,Opa1andAtad3aafterthetransfectionandinterferenceofthreebenzenephosphoruscompound;ATPBioluminescenceAssayKitwasusedtoanalyzeATPcontentinA549cellspheresandA549cellafterthetransfectionandinter
4、ferenceofthreebenzenephosphoruscompound;Flowcytometrytestapoptosicrateandmitochondrialmembranepotentialinallgroups.Result:ThemRNAandproteinexpressionofmitochondriafusionrelatedproteinMfn1,Mfn2,Opa1andAtad3ainA549cellspheresincreasedsignificantly;MitochondrialdistributioninA549cellsphere
5、saroundthenuclearandmitochondrialmembranepotentialintheA549cellspheresarehigher;Afterpeptidenucleicacid-threebenzenephosphoruscompoundsactedonmitochondriafusioninA549cellspheresandA549cell,theexpressionofmitochondrialfusionrelatedproteinMfn1,Mfn2,Opa1decreased,whiletheexpressionofAtad3a
6、isnochange,atthesametimetheATPcontentwereallreduced,thecellapoptosisrateincreasedsignificantlyandthemitochondrialmembranepotentialdecreasedobviously.Conclusion:1.ThemRNAandproteinexpressionofmitochondriafusionrelatedproteinMfn1,Mfn2,Opa1andAtad3ainA549cellspheresincreasedsignificantlyth
7、aninA549cells,peptidenucleicacid-threebenzenephosphoruscompoundstargetinginhibitmitochondrialfusionenhancesthesensitivityofcisplatinchemotherapy.2.Peptidenucleicacid-threebenzenephosphoruscompoundsaccumulationinA549lungcancercellspheresastheirhigh?ψm,closedgeneexpressionofmtDNA
