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《mir-21調(diào)控舌鱗癌細(xì)胞凋亡的實驗研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、中山大學(xué)博士學(xué)位論文miR．2l調(diào)控舌鱗癌細(xì)胞凋亡的實驗研究However,whatisthebiologymechanismforgeneregulationandexpressionchangeintheprocessofTSCC?Althoughnocertainresult，non·codingRNAs，especiallymicroRNAs(miRNAs)，inspiretheresearchers．Itisknownthattumorigenesisreferstomultiplegenev
2、ariationandsinglegeneinterventioncannotinhabittumoranditsprogress．AsendogenousmiRNAcanregulateafeworhundredsgenes；besides，thosemiRNAsaremuchlessthanthecodinggenes，theyshouldbethestableandeffectiveanti—tumortargets．StudieshaveindicatedthatmiRNAsparticipat
3、einaseriallifeprocess，includinggrowth，hematopoiesis，organ—formation，apoptosis，cellproliferationandtumorigenesis．Theoncogeneoranti—oncogenemiRNAscorrelatewithcellproliferation，apoptosisanddifferentiation．Recently,highthroughputmicroarrayassayandquantitati
4、verealtimeRT-PCR(qRT-PCR)inresearchofcoloncancer,breastcancer,lungcancer，renalcancer，gastriccancer，lymphoma，leukemia,eta1．revealmiRNAsprofileofcancersandthechangesfromtheoriginallynormaltissues．Researchesindicatethattumors’malignancypositivelycorrelatedw
5、iththemiRNAchangeandinsomeadvancedcancer’certainmiRNAexpressionlevelsareevenlower．Inbreastcancer，miR一10b，let一7，miR一125bandmiR-145aredown-regulated，whilesomemiRNAsareup-regulated，likemiR一21andmiR一155．ThosealterationsofmiRNAsintumorsuggesttheeffectsofgener
6、egulation．ForheadandneckcancelparticularlytheTSCC，miRNAsandtheirfunctionsaleseldomstudied．WeemployedhighthroughputmicroarrayassaytoscreendifferentialexpressionofendogenousmiRNAsinTSCCtissuesofdifferentclinicalstagesandnormaltissue．ThenwefocusedonthemiR一2
7、1whichhassignificantlyhighexpressioninbothearlyandadvancedTSCC．Wecollected50independentsamplesofTSCCpatientsofdifferentclinicalstagesandusedqRT-PCRtotestifythedifferentialexpressionofmiR一21．Meanwhile，weusedimmunohistochemistrytechnologytodeterminetheexpr
8、essionoftropomyosin—I(TPM—1)，thetargetofmiR一21，inthesame50samples．AndwedetectedcellapoptosisbyTunelapoptosisdetectionkit．Combinedwithpatients’clinicdata，weanalyzedtherelationshipamongthoseindexes．Basedonthoseresults—VIII—中
