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1、遵義醫(yī)學(xué)院碩士學(xué)位論文阻斷吡那地爾后處理大鼠心肌線粒體比較蛋白質(zhì)組學(xué)的研究reliabilityof2一DE．Results：1．ThepurifiedmyocardialmitochondriaisolatedbyNycodenzdensitygradientcentrifugationwereprovedquitewellthroughwesternblotanalysis．2．Thecardiacfunction：Therewasnoevidentchangeeachotherattheendpointofequilibration．Afterreperf
2、usion，GroupNorandGroupPinawasobviouslybetterthanGroupI／Rand5HD+Pina(P<0．05)．TherewasnosignificantdifferencebetweenGroup5HD+PinaandGroupI／R．3．Obtainedwellfocuseanddistinct2-DEmaps，meansof485+22proteinspotsweredetectedineachgroup．Therewere9proteinspotsthattheirdifferenceexceed2times，an
3、dthesedifferentialexpressionproteinsweresuccessfullyidentifiedbyMS．4．TheexpressionlevelsofATPAandNDUFA10weredecreased，whilethoseofACADL，NDUFS2，NDUFA10，ALDH2，ODP2andIMMTwereincreasedinGroup5-HD+PinacomparedwithGroupPina．Interestingly,threespotsinthe2-DEmapwereidentifiedasNDUFAl0．5．Wes
4、ternblotverification；TheexpressionofNDUFAl0，NDUFS2andALDH2areincreased，buttheATPAisdecreasedinGroup5HD+Pina．Conlusion：1．Pinacidilpostconditioningcanobviouslyimprovetheratheartfunction，whiletheeffectioncanbeantagonizedby5-hydroxydecanoate．．2．5-HDantagonizepinacidilpostconditioningbloc
5、kthemitochondrialATP—sensitivepotassiumchannel，probablyleadingtothefollowingresults：①TheexpressionlevelsofATPAisdecreasedafter5HD+Pin亂whichisrelatedtotheabnormalsynthesisandtransportprocesses．②TheexpressionofACADL，NDUFS2，ALDH2，ODP2andIMMTareincreased，whichcanresultfromthecompensatory
6、mechanism．③NDUFAIOmaybeshearedormodifiedwhenmitochondrialATP—sensitivepotassiumchannelwasantagonized．3．UtilizingNycodenzdensitygradientcentrifugationafterdifferentialcentrifugationcanobtainmyocardialmitochondriawithhigherpurity．KeyWords：5-hydroxydecanote：Pinacidil；Postconditioning；Mi
7、tochondria；Proteomics：Two—dimensionalelectrophoresis；Myocardium——●、——遵義醫(yī)學(xué)院碩士學(xué)位論文阻斷吡那地爾后處理大鼠心肌線粒體比較蛋白質(zhì)組學(xué)的研究．iL—．L目IJ吾缺血再灌注損傷(ischemicreperfusioninjury，I／R)是當(dāng)今心肌保護(hù)研究的熱點(diǎn)，近年來集中于通過缺血后處理(ischemicpostconditioning，IPTC)以激發(fā)機(jī)體內(nèi)源性抗再灌注損傷能力。Staat[1]首次將缺血后處理應(yīng)用于臨床冠脈成形術(shù)來治療進(jìn)行性急性心肌缺血，收到了較好的心臟保護(hù)作用。然而，缺
8、血后處理存在著反復(fù)嵌閉血
