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1、山西醫(yī)科大學碩士學位論文睪酮誘導骨髓巨噬細胞凋亡并增加杜氏利什曼原蟲對巨噬細胞的侵襲力姓名：劉立民申請學位級別：碩士專業(yè)：病原生物學指導教師：喬中東2002.5.6艮；7鍋晦籮性j彬蚺¨礱7廣薌和西矽乏癱菩專{侈{f，矽山≯撒強印蒯一向譬U啶認知心嗲從季弘石繹瞄、毫)薦能I司：狙繼例色謦縫挫疑哆畢竺彌鑼藐徽新形p呔秀厥橢彬讎Ⅳ統(tǒng)”r。丫“，、，彤一～●厶文＼∞榔罄ShanxiMedicalUniversityAcademicDissertationforMaster_——_————●—————————-————_—————————-—————————————————————————
2、———一一AbbreviationsRTARBMMbpca2+hDEPCDNAEBEDTAFCMFCSgGPCRTLCMLPS上．DMreversetranscriptaseandrogenreceptorBoneMarrowderived—Macrophagebasepair(s)ionizedcalciumhourdiethylpyrocarbonatedeoxyribonucleicacidethidiumbromideethylenediaminetetraaceticacidflowcytometerfetalcalfsei3．1mgramorgravityG·-prot
3、ein·-coupledreceptorsfortestosteroneL929eellconditionedmediumlipopolysaccharideLeishmaniadonovanfmole／LShanxiMedicaJIjniversityAcademicI)issertalion}0rMasterM—CSFmMmRNAOD260pBSPCDpCRPIRNARNaseSDSTB毯TeE2TE鏘．伐1’isUVHREmacrophage&monocyte—colonystimulatingfactormillimole／Lmessengerribonucleicacid
4、opticaldensityat260nmphosphate—bufferedsalineProgrammedCellDeathPolymeraseChainReactionpropidiumiodideribonucleicacidribonucleasesodiumdodecylsulfateTris／borateelectrophoresis(buffer)testosteronel7．B—estradioltumornecrosisfactor-atris(hydroxymethyl)aminomethaneultra．violethormoneresponseelemen
5、tShanxiMedicalUniversityAcademicDissertationIbrMasterAbstractSexualdimorphismintheimmunesystemsoffemalesandmalesiswellestablished．Thesex—dependentdifferencesbetweenfemaleandmalenormalandautoimmuneresponsesisgenerallyattributedtotheinfluenceofestrogensandandrogenseventhoughlimitedinformationhas
6、beenreportedonthespecificmechanismsofsteroidsonimmunecells．Ingeneraltermsfemaleshaveagreaterresistancetovariousbacterialandviralinfectionsthanmaleswiththeirotherenhancedimmunecapabilitiesmightcontributetotheirhighersurvivalrates．Macrophagesareversatilecellswhoseactivitiesareprogrammedbyenviron
7、mentalsignalsTheevidenceaccumulatedtodatesupportsthepostulatethatsexhormonesprofoundlyinfluencehostdefensebycontrollingtheabilityofmacrophagestoparticipateinimmuneresponses．Inthepresentstudy,weinvestigatedthedirecteffectofsexhormonesonm
