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1、骨髓巨核細(xì)胞特異染色法及臨床應(yīng)用的研究摘要目的：對血液痘中骨髓巨核細(xì)胞形態(tài)學(xué)進(jìn)行研究，為血液病的診斷和鑒別診斷提供新方法和新思路。方法：選擇24種染料配成不同溶劑的染液，對血細(xì)胞進(jìn)行單二染色，評價其形態(tài)學(xué)特征。同時與流式細(xì)胞技術(shù)及細(xì)胞化學(xué)染色技術(shù)對比觀察。(結(jié)果(1)正常和血液病人的骨髓或血涂片經(jīng)堿性品藍(lán)雙、染色后，原始巨核細(xì)胞、幼稚巨核細(xì)胞、巨核細(xì)胞的核染成亮藍(lán)色，胞漿染成紫紅色，血小板染成紫紅色，小巨核細(xì)胞的核染成亮藍(lán)色，胞漿染成紫紅色。(2)同樣的方法染色，原始粒細(xì)胞核染為淡藍(lán)紫色，胞漿為深藍(lán)色；早幼粒細(xì)胞核染為淡藍(lán)紫色，漿染為深藍(lán)色：中、
2、晚幼粒細(xì)胞核均染為淡紫藍(lán)色，漿染為淡藍(lán)色；桿狀核、分葉核粒細(xì)胞核染為淡紫藍(lán)色，胞漿染為淡藍(lán)粉色；嗜堿粒細(xì)胞核染為紫紅色，漿染為淡粉色，嗜堿性顆粒染為深紫色，嗜酸粒細(xì)胞顆粒不著色。(3)單核細(xì)胞、淋巴細(xì)胞核漿均染為淡藍(lán)色；漿細(xì)胞核染為淡藍(lán)紫色，胞漿染為深藍(lán)色，近核區(qū)為紫紅色。(4)原紅細(xì)胞核染為淡藍(lán)紫色，核仁為天藍(lán)色，胞漿染成深藍(lán)色；早幼紅細(xì)胞核染為淡紫紅色，胞漿染為深藍(lán)色；中幼紅細(xì)胞核染為藍(lán)綠色，核上有紫紅色絲網(wǎng)分布，胞漿染為深藍(lán)色；晚幼紅細(xì)胞核同中幼紅細(xì)胞，胞漿染為棕紅色，成熟紅細(xì)胞染為棕紅色。通過與其它組化染色方法比較及用流式細(xì)胞儀法鑒定，j
3、正明堿性品藍(lán)雙染色法對巨核細(xì)胞染色是可靠而特異的。)結(jié)論：國產(chǎn)紡織染料堿性品藍(lán)作為一種在光學(xué)顯微鏡下識別巨核細(xì)胞的染料，具有特異性高，染色方法簡便快速等優(yōu)點(diǎn)。采用堿性品藍(lán)雙染色染巨核細(xì)胞在血液病診斷和鑒別診斷上具有實用價值。且染料便宜易得，易于在臨床推廣應(yīng)用。關(guān)鍵詞形態(tài)學(xué)堿性品藍(lán)巨核細(xì)胞染色TheStudyandtheClinicalUseofDistinctiveStainofMegkaryocytesinBoneMarrowAbstractObject：Tostudythemorphologyofmegkaryocytesinbonemarr
4、owandtoprovideanewmethodandnewideafordiagnosisanddifferentialdiagnosisofblooddisease．Methods：Twenty-fourkindsofweavingdyesweredissovledindifferentsolvent，whichwasusedforstaintoevaluatethemorphologicfeaturesofbloodcells．Results：Thenuclearofmegakaryocytewasstainedpurpleredandfr
5、equentlycytoplasmwasstainedbrightblue．Inmyeloblastsandpromyelocytes，nucleiwerestainedpaleblueandthecytoplasmstaineddeepblue．Inneutrophilicmyetocytesandneutrophilicmetamyelocytes，nucleiwerestainedpalepurpleandthecytoplasmstainedpaleblue．Inmaturegranulocytes，nucleiwerestainedpa
6、lepurpleandthecytoplasmstainedpaleblue．Inbasophils，granuleswerestaineddeeppurpleandnucleiwerefaintypurple．Ineosophils，nucleiwerefaintlypurpleandgranulesstainednothing．Innormalandleukemicmonocytesandlymphocytes，nucleiandcytoplasmawereallpaleblue．Inplasmacytes，nucleiwerestained
7、paleblueandplasmstaineddeepbluewhilepurple‘redwereseennearnuclerregion．Inpronormoblast，nucleiwerestainedpaleblue-purple，nucleoliwerebrightskyblue，andplasmstaineddeepblue．Inearlvnormoblast，nucleiwerestainedpaleredpurpleandplasmstaineddeepblue．Inpolychromaticnormoblast，nucleiwe
8、re1stainedbrightblueandoftenshowedaretia—likepatternofred—·purplemat
