資源描述：
《大理石紋蝎多肽毒素的基因克隆、原核表達與純化研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、分類號Q819學(xué)號14020052ＵＤＣ606密級公開工學(xué)碩士學(xué)位論文大理石紋蝎多肽毒素的基因克隆、原核表達與純化研究碩士生姓名姚夢學(xué)科專業(yè)生物醫(yī)學(xué)工程研究方向生物活性多肽毒素指導(dǎo)教師張東裔教授國防科學(xué)技術(shù)大學(xué)研究生院二〇一六年十一月GeneCloning,ProkaryoticExpressionandPurificationoftoxinsfromScorpionLychasmucronatusCandidate：YaoMengAdvisor：ZhangDongyiAdissertationSubmittedinpartialful
2、fillmentoftherequirementsforthedegreeofMasterofEngineeringinBiomedicalEngineeringGraduateSchoolofNationalUniversityofDefenseTechnologyChangsha，Hunan，P.R.China（November，2016）國防科學(xué)技術(shù)大學(xué)研究生院碩士學(xué)位論文目錄摘要............................................................................
3、.....................................iABSTRACT.........................................................................................................ii第一章緒論......................................................................................................11.1多肽毒素基因克
4、隆、異源表達與純化的研究進展.....................................11.1.1多肽毒素基因克隆的研究進展.............................................................21.1.2多肽類毒素異源表達研究進展............................................................51.1.3多肽毒素分離純化的研究進展..........................................
5、................101.2本文的研究背景和思路.................................................................................12第二章利用大腸桿菌表達系統(tǒng)表達兩種大理石紋蝎多肽毒素.........................152.1前言...................................................................................................
6、..............152.2實驗器材與試劑準(zhǔn)備.....................................................................................162.2.1實驗器材..............................................................................................162.2.2實驗材料..........................................
7、....................................................162.2.3部分溶液、試劑的制備......................................................................172.3實驗方法與步驟.............................................................................................192.3.1設(shè)計構(gòu)建重組的表達質(zhì)粒pET-His-S
8、UMO-GT028649和pET-His-SUMO-GT028758.....................................................................
