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《NMDA受體與AMPA受體亞單位在培養(yǎng)海馬神經(jīng)元樹突上的表達(dá)與共定位》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、漸江大學(xué)醫(yī)學(xué)院碩士論文懶受體與^If^受體在培養(yǎng)海馬神經(jīng)元樹突上的表達(dá)及共定位SurfaceexpressionandcoIocalizationofNMDArecptorandAMPAreceptorOndendmesofculturedhippocampalneuronszhcjianguniVers時schoolofMedicine：cliIlicalmedicinePost礦aduateforMasterDegreeinNeurology：HuangManSupeⅣisors：DingMei-PingLuoJi衄-HongAbsn·actAcen仃alassumlmoninneur
2、obiologyhol如thatsyn印sefo衄ationaIlds”apticplastic姆llIlderliethecreationoffIlnctionalneuralcircuitsandnervoussystemplastici吼T鉀omajorsub呻esofiono仃opicglutaI】怵ercceptorS(G1uRs)，N．methyl—D-嬲p甜乜te(NMDA)一typeand∞砌ino一3-hydroxy-5-methyl一4-isoxaZol印mpionate(AMPA)-typereceptors，arcfoundlocalizedonglut鋤ate唱i
3、cpostsyna砸cexcitatorysyn印se．ThreegenefhmiliesmatencodeNMDAreceptorsubunitshavebeenide塒fied：NRl，NR2A，alldNR3subllll婦AM隊(duì)receptorsubllnitshaVebeenidentifjed：GuRl、GluR2、GluR3、GIuR4subllnitsInourstlldy·獅oVectorsexpressinggreennuorescentpmteinN-teminally協(xié)ggedGluRlsubllnit(GFP—GluRl)／GluR2subunit(GFP—Glu
4、R2)andFLAGN—te舯inally詛ggedNR2Bsubunit(FLAG—NR2B)wercgcncratcdand咖nsfectedintoculnlredhippocampalneuro璐at5daysinVi仃D(DIV5)．surfaceexpressedFLAG-NR2Bcon乜iningNMDArec印torclusters髓dGFP—GluRlcon協(xié)iningAMPAmceptorclusters／(扦P-GluR2containingAMP：AreceptorclusterswerethenlabeledinIivingneIlronsbyusing鋤ti—F
5、LAGprimaryantibodyfollowedbyCy3一conjugatedsecondaryamibodyandanti—GFPprinlaIyantibodyfollowedby5浙江大學(xué)醫(yī)學(xué)院碩士論文N岫^受體與^樅受體在培養(yǎng)海馬博經(jīng)元樹突上的裹達(dá)及共定位Alex488一conjugatedsccondaryall曲ody．Funhemlore，dis仃ibutionoft量le礬】嘞ceclustersⅥ，asobservedonthedetailedde枷tics仃ucnJrcsVisualizedwithco—expressionofcyanfluorescentpro
6、teintaggedActin(CFP—Acdn)．wefindthat：1)Si印ificantdi仃erencewasfoundbothillNR2BsubllnitscontainingNMDArec印torandGluRlsubunitscontainingAMPArcc印torbe俯eenDⅣ7aIldDIvl4C010calization曲c舶nofNR2BsllbIln砥containingNMDAreceptorandthatofGluRlsubunitscontainingAMPArcceptorwerebomincreasingwilhmedevelopmentofne
7、llrons．hlmatIlreneumns，70％rec印torclusterslocatedatthedendriticspiIleswerein也eformofcolocalization，髓d40％oVedaidclustcrswereIocatedat血edend—ticspines．2)Densit)rofGluR2subunitscon詛“ngAM隊(duì)receptor(GluR2-AM隊(duì)R)wereincre
