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1、ThePlantJournal(2007)52,1001–1013doi:10.1111/j.1365-313X.2007.03291.xGeneticcharacterizationrevealsnoroleforthereportedABAreceptor,GCR2,inABAcontrolofseedgerminationandearlyseedlingdevelopmentinArabidopsis1,2,?1,?11,331,*YajunGao,QingningZeng,JianjunGuo,JiaCheng,BrianE.EllisandJin-GuiChen1Dep
2、artmentofBotany,UniversityofBritishColumbia,Vancouver,BCV6T1Z4Canada,2CollegeofResourcesandEnvironment,NorthwestA&FUniversity,Yangling,Shaanxi712100,China,and3MichaelSmithLaboratories,UniversityofBritishColumbia,Vancouver,BCV6T1Z4,CanadaReceived3May2007;revised30July2007;accepted1August2007.*
3、Forcorrespondence(fax+16048226089;e-mailjingui@interchange.ubc.ca).?Theseauthorscontributedequallytothiswork.SummaryAbscisicacid(ABA)isperceivedbyseveraldifferenttypesofreceptorsinplantcells.Atthecellsurface,theABAsignalisproposedtobeperceivedbyGCR2,whichmediatesABAresponsesinseedgermination,
4、earlyseedlingdevelopmentandstomatalmovement.GCR2wasalsoproposedtobeaseven-transmembrane(7TM)G-protein-coupledreceptor(GPCR).HerewecharacterizeGCR2andoneofitstwohomologs,GCR2-LIKE1(GCL1),inABA-mediatedseedgerminationandearlyseedlingdevelopmentinArabidopsis.Weshowthatloss-of-functionmutationsin
5、GCL1didnotconferABAinsensitivity.Similarly,wedidnotobserveABAinsensitivityinthreeindependentgcr2alleles.Furthermore,wegeneratedgcr2gcl1doublemutantsandfoundthatthedoublemutantsstillhadnearwild-typeresponsestoABA.Consistentwiththis,wefoundthatthetranscriptionofABAmarkergeneswasinducedbyABAtole
6、velsthatwerecomparableinwildtypeandgcr2andgcl1singleanddoublemutants.Ontheotherhand,theloss-of-functionallelesofthesoleArabidopsisheterotrimericGproteinasubunit,GPA1,werehypersensitivetoABAintheABA-inhibitionofseedgerminationandearlyseedlingdevelopment,disfavoringageneticcouplingofGCR2byGPA1.
7、Usingmultiplerobusttransmembranepredictionsystems,GCR2waspredictednottobea7TMprotein,astructuralhallmarkofGPCRs.Takentogether,ourresultsdonotsupportthenotionthatGCR2isanABA-signalingGPCRinseedgerminationandearlyseedlingdevelopment.Keywords:7T
