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《N乙酰半胱氨酸對大鼠重癥急性胰腺炎腸黏膜屏障的作用》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫。
1、中文摘要目的探討早期應(yīng)用N-乙酰半胱氨酸（N-acetylcysteine,NAC）對實驗性大鼠重癥急性胰腺炎（severeacutepancreatitis,SAP）腸黏膜屏障的作用。方法采用大鼠兩次腹腔內(nèi)注射大量L?精氨酸（L-Arginine）溶液誘導(dǎo)大鼠SAP模型。48只Wistar大鼠隨機分為4組：正常組（A）.急性胰腺炎組（B）、生理鹽水對照組（C）、NAC處理組（D）,每組12只。NAC處理組于造模前l(fā)h腹腔內(nèi)注射10%NAC溶液，劑量為300mgAcg；生理鹽水對照組則于造模前l(fā)h腹腔內(nèi)注
2、射生理鹽水，劑量為300mg/kg。各組大鼠在造模成功后于24h和48h分批處死，每組每批處死6只。觀察血清淀粉酶、白介素一6（IL-6）和乳酸（LD）水平，血漿內(nèi)毒素水平，小腸黏膜勻漿的丙二醛（MDA）水平，胰腺和腸黏膜病理學(xué)變化。結(jié)果與A組比，B組的血清淀粉酶和IL4水平明顯升高，有顯著差異；胰腺組織浸潤、水腫.出血、壞死明顯，小腸黏膜組織水腫.絨毛脫落明顯。與B組和C組比，D組胰腺組織的岀血.壞死程度減輕；小腸黏膜水腫.絨毛脫落程度減輕；血清淀粉酶、IL?6和內(nèi)毒素水平以及小腸黏膜組織的MDA水平有
3、所下降。結(jié)論SAP大鼠早期即存在腸黏膜屏障的損害；早期應(yīng)用NAC可以有效地減輕SAP時腸黏膜屏障的病理損傷，降低門靜脈的內(nèi)毒素水平：進而可以延緩SAP病情的發(fā)展。關(guān)鍵詞急性胰腺炎抗氧化劑腸黏膜屏障EffectofN-acetylcysteineontheintestinalmucosabarrierinratswithsevereacutepancreatitisAbstract■ObjectiveToinvestigatetheeffectofN-acetylcysteineontheintestina
4、lmucosabarrierinratswithsevereacutepancreatitis(SAP).MethodsSAPratswereinducedbyintraperitonealadministrationofahighdoseofL-arginine(250mg/lOOgbm,twiceatanintervaloflh).Forty-eightmaleMstarratsweredividedintofourgroupsrandomly(n=12),includingnormalgroup(g
5、roupA),SAPgroup(groupB),NaClcontrolgroup(groupC),NACtreatmentgroup(groupD).NAC-treatmentg!：oxpwasgiven300mg/kgNACintraperitoneallylhbeforeinducingSAP.NaClcontrolgroupwasgiven300mg/kgNaClintraperitoneallylhbeforeinducingSAP.RatswerekilledafterthefirstL?Ar
6、ginjection?Eachgroupwasexaminedthecontentsofserumamylase*endotoxin?D-lactate>interleukin-6.ThelevelofNOandMDAinrat'sintestinalmucosatissuesampleofeachgroupwereassayed?Thepathologicchangesofpancreatictissueandintestinaltissuewereobservedundermicroscopes.Re
7、sultsIngroupB,thelevelofamylaseandIL-6werehigherthanthoseinthegroupA;thepathologicchangesofpancreatictissueat24h,48haftertheinjectionofL-ArginineweresignificantlysevererthanthoseingroupA.ComparedwithgroupBandgroupC,thecontentsofamylase,IL?6,endotoxinandMD
8、Aweresignificantlylower;ThehistopathologicalchangesofpancreaticandintestinalmucosawererelievedingroupD.ConclusionTherewasinjuryoftheintestinalmucosabarrierintheearlystageofrat'sSAP.EarlyNACtreatmentcancureSAP-associ
