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《復(fù)發(fā)性流產(chǎn)患者外周血調(diào)節(jié)性T細(xì)胞檢測(cè)及臨床意義》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、復(fù)發(fā)性流產(chǎn)患者外周血調(diào)節(jié)性T細(xì)胞檢測(cè)及臨床意義作者：李成,張強(qiáng)單位：【摘要】目的探討不明原因復(fù)發(fā)性流產(chǎn)患者外周血以CD4+CD25high-intCD127lowTreg(CD4+CD25high-intCD127lowTregularcells)為代表的調(diào)節(jié)性T細(xì)胞表達(dá)及其意義。方法采用三色熒光抗體標(biāo)記及流式細(xì)胞術(shù)分析20例不明原因復(fù)發(fā)性流產(chǎn)患者、正常妊娠組和正常非孕對(duì)照組外周血CD4+CD25high-intCD127lowTreg占CD4+T淋巴細(xì)胞的比例。結(jié)果正常孕婦外周血CD4+CD25high-intCD127lowT
2、reg較正常對(duì)照組差異有統(tǒng)計(jì)學(xué)意義（P<0.01），復(fù)發(fā)性流產(chǎn)患者CD4+CD25high-intCD127lowTreg調(diào)節(jié)性T細(xì)胞較正常對(duì)照組差異有統(tǒng)計(jì)學(xué)意義（P<0.01）,正常孕婦組較復(fù)發(fā)性流產(chǎn)患者組差別則更顯著；其中流產(chǎn)次數(shù)小于或等于3次的患者與流產(chǎn)次數(shù)大于或等于4次的患者其外周血CD4+CD25high-intCD127lowTreg占CD4+T細(xì)胞百分比差異無統(tǒng)計(jì)學(xué)意義(P>0.05)。結(jié)論原因不明復(fù)發(fā)性流產(chǎn)患者外周血中調(diào)節(jié)T細(xì)胞比例降低，這種改變導(dǎo)致原因不明復(fù)發(fā)性流產(chǎn)患者細(xì)胞免疫耐受被打破，可能是
3、原因不明復(fù)發(fā)性流產(chǎn)患者發(fā)病的重要原因之一?！娟P(guān)鍵詞】復(fù)發(fā)性流產(chǎn)；調(diào)節(jié)性T細(xì)胞；流式細(xì)胞術(shù)SignificanceofmeasurementofregulationTcellsinperipheralbloodofpatientswithrecurrentspontaneousabortionLiCheng，ZhangQiangDepartmentofClinicalLaboratory,BengbuSecondPeoples'sHospital,Bengbu233000［Abstract］ObjectiveToinvestigat
4、etheexpressionandsignificanceofCD4+CD25high-intCD127lowTreg(CD4+CD25high-intCD127lowTRegularCells)intheperipheralblood(PB)frompatientswithunexplainedrecurrentspontaneousabortion(URSA),toexplorethemechanismofautoimmuneintoleranceinURSA.MethodsTheproportionofCD4+CD25hig
5、h-intCD127lowTreginthePBofthreegroupsofpeople(URSApatientsthattheyhadbeendiagnosedclearly,normalnon-pregnantwomenandthenormalpregnantwoman)analyzedbytriadcolourfluoresce-labeledmonoclonalantibodyandusingmultiparameterflowcytometry.ResultsTheproportionofCD4+CD25high-in
6、tCD127lowTreg/TCD4+cellsinthenormalpregnantwomanwassignificantlyhigherhtanthoseincontrolgroup(P<0.01);theproportionofCD4+CD25high-intCD127lowTreg/TCD4+cellsinURSApatientswassignificantlylowerthanthoseincontrolgroup(P<0.01).ConclusionThepercentagesofCD4+CD25high-
7、intCD127lowTregwasdecreased,whichbreakdowntheautoimmunetoleranceinURSA,thesechangesofCD4+CD25high-intCD127lowTreginnumbermightparticipateinonsetofURSA,itmaybecorrelatedtopathogenesisofURSA.［Keywords］Recurrentspontaneousabortion;RegulatoryTcell;Flowcytometry近代生殖免疫學(xué)研究認(rèn)為
8、，正常妊娠是一個(gè)類似于同種異體移植的復(fù)雜生理過程，并逐步認(rèn)識(shí)到胚胎攜帶1/2有異于母體的組織抗原，然而不被母體免疫系統(tǒng)排斥的實(shí)質(zhì)是依賴于母胎之間的免疫耐受關(guān)系，這一耐受一旦失調(diào)，從而導(dǎo)致母胎免疫失衡，將可能發(fā)生包括原因不明復(fù)發(fā)性流產(chǎn)在內(nèi)的病理妊娠，
